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Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro
Xiong, Chuannan1,3; Wu, Haige1,2; Wei, Peng1,3; Pan, Ma1,3; Tuo, Yaqin1; Kusakabe, Isao4; Du, Yuguang1; Du YG(杜昱光)
KeywordDeacetylated Chitohexaose Angiogenesis Ecv304 Vascular Endothelial Growth Factor Urokinase Plasminogen Activator
Source PublicationCARBOHYDRATE RESEARCH
2009-10-12
ISSNISSN: 0008-6215
DOI10.1016/j.carres.2009.06.036
Volume344Issue:15Pages:1975-1983
Indexed BySCI
SubtypeArticle
Department18
Funding Project1805
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Chemistry, Organic
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordENDOTHELIAL GROWTH-FACTOR ; CHICK CHORIOALLANTOIC MEMBRANE ; MATRIX METALLOPROTEINASES ; PHYTOPHTHORA-CAPSICI ; TUMOR ANGIOGENESIS ; ANTI-ANGIOGENESIS ; OLIGOCHITOSAN ; CELLS ; INDUCTION ; STRATEGIES
AbstractThis study was performed to demonstrate the effects of deacetylated chitohexaose (hexamer) separated from a chitooligosaccharide (COS) mixture on tumor angiogenesis and its mechanism of action. Five fractions from dimer to hexamer were separated by a linear gradient solution of HCl on a cation-exchange resin. Then HCl was removed from the fractions by a charcoal column. The purity of the five fractions was analyzed by HPLC and the molecular masses were analyzed by MALDI-TOFMS. The hexamer expressed an inhibitory influence on CAM angiogenesis in a dose-dependent manner at concentrations of 6.25-50 mu g/egg. On further investigation, we found that the hexamer had no toxic effect on normal ECV304 cells, but could inhibit the proliferation and migration of tumor-induced ECV304 cells in a dose-dependent manner. The mechanism was demonstrated through the detection of mRNA expression of VEGF, MMP-9, TIMP-1, TIMP-2 and uPA by RT-PCR, which showed that the hexamer down-regulated the VEGF and uPA mRNA expressions in ECV304 cells, but up-regulated the TIMP-1 mRNA expression. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000271043300007
Citation statistics
Cited Times:43[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/101905
Collection中国科学院大连化学物理研究所
Corresponding AuthorDu YG(杜昱光)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ, Bioengn Coll, Dalian 116622, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
4.Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 305, Japan
Recommended Citation
GB/T 7714
Xiong, Chuannan,Wu, Haige,Wei, Peng,et al. Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro[J]. CARBOHYDRATE RESEARCH,2009,344(15):1975-1983.
APA Xiong, Chuannan.,Wu, Haige.,Wei, Peng.,Pan, Ma.,Tuo, Yaqin.,...&杜昱光.(2009).Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro.CARBOHYDRATE RESEARCH,344(15),1975-1983.
MLA Xiong, Chuannan,et al."Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro".CARBOHYDRATE RESEARCH 344.15(2009):1975-1983.
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