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题名: Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry
作者: Yang, Li1;  Wang, Yun1;  Wang, Longxing2;  Xiao, Hongbin2;  Wang, Zhengtao1;  Hu, Zhibi1
通讯作者: Zhengtao Wang
关键词: Iridoid glycosides ;  Longdan Xiegan Decoction ;  Gentianae Radix ;  Gardeniae Fructus ;  High-performance liquid chromatography ;  Electrospray ionization mass spectrometry ;  Multiple reaction monitoring (MRM)
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-03-13
DOI: 10.1016/j.chroma.2008.06.043
卷: 1216, 期:11, 页:2098-2103
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1804
产权排名: 2;3
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Longdan Xiegan Decoction (LXD) is a formulated preparation composed of 10 ingredient herbs, with iridoids as the main bioactive components. In this study, a rapid, simple and reliable method of simultaneous determination of four iridoid glycosides in LXD using high-performance liquid chromatography (HPLC) coupled with electrospray ionization mass spectrometry (MS) was first developed and validated. The four iridoid glycosides references were isolated from LXD extract and purified using a preparative HPLC chromatography. The sample preparation for quantification comprised of a simple ultrasonic extraction and the satisfactory chromatographic separation of the four structurally similar iridoid glycosides was effected in less than three minutes on a CAPCELL PAK C(18) MGII column (3 mu m, 100 mm x 2.0 mm), using an elution system of 10% methanol and their concentrations in different batches of LXD and ingredient herbs were simultaneously determined by HPLC-MS/MS using a multiple reaction monitoring (MRM) mode. The method was validated with respect to the overall intra- and inter-day variation (RSD less than 8%) and the limits of quantification for the four iridoid glycosides were 35, 20. 37 and 33 ng/mL, respectively. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: DIODE-ARRAY DETECTION ;  QUANTITATIVE-DETERMINATION ;  FINGERPRINTING ANALYSIS ;  SECOIRIDOID GLUCOSIDES ;  HPLC ;  SPECTROSCOPY ;  GENIPOSIDE ;  EXTRACTS ;  PLANTS ;  ESI/MS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000264226500015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101917
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Univ Tradit Chinese Med, Key Lab Standardizat Chinese Med, Minist Edu, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Li,Wang, Yun,Wang, Longxing,et al. Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2098-2103.
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