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Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry
Yang, Li1; Wang, Yun1; Wang, Longxing2; Xiao, Hongbin2; Wang, Zhengtao1; Hu, Zhibi1; Zhengtao Wang
KeywordIridoid Glycosides Longdan Xiegan Decoction Gentianae Radix Gardeniae Fructus High-performance Liquid Chromatography Electrospray Ionization Mass Spectrometry Multiple Reaction Monitoring (Mrm)
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2009-03-13
DOI10.1016/j.chroma.2008.06.043
Volume1216Issue:11Pages:2098-2103
Indexed BySCI
SubtypeArticle
Department18
Funding Project1804
Contribution Rank2;3
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordDIODE-ARRAY DETECTION ; QUANTITATIVE-DETERMINATION ; FINGERPRINTING ANALYSIS ; SECOIRIDOID GLUCOSIDES ; HPLC ; SPECTROSCOPY ; GENIPOSIDE ; EXTRACTS ; PLANTS ; ESI/MS
AbstractLongdan Xiegan Decoction (LXD) is a formulated preparation composed of 10 ingredient herbs, with iridoids as the main bioactive components. In this study, a rapid, simple and reliable method of simultaneous determination of four iridoid glycosides in LXD using high-performance liquid chromatography (HPLC) coupled with electrospray ionization mass spectrometry (MS) was first developed and validated. The four iridoid glycosides references were isolated from LXD extract and purified using a preparative HPLC chromatography. The sample preparation for quantification comprised of a simple ultrasonic extraction and the satisfactory chromatographic separation of the four structurally similar iridoid glycosides was effected in less than three minutes on a CAPCELL PAK C(18) MGII column (3 mu m, 100 mm x 2.0 mm), using an elution system of 10% methanol and their concentrations in different batches of LXD and ingredient herbs were simultaneously determined by HPLC-MS/MS using a multiple reaction monitoring (MRM) mode. The method was validated with respect to the overall intra- and inter-day variation (RSD less than 8%) and the limits of quantification for the four iridoid glycosides were 35, 20. 37 and 33 ng/mL, respectively. (C) 2008 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000264226500015
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/101917
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhengtao Wang
Affiliation1.Shanghai Univ Tradit Chinese Med, Key Lab Standardizat Chinese Med, Minist Edu, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yang, Li,Wang, Yun,Wang, Longxing,et al. Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2098-2103.
APA Yang, Li.,Wang, Yun.,Wang, Longxing.,Xiao, Hongbin.,Wang, Zhengtao.,...&Zhengtao Wang.(2009).Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry.JOURNAL OF CHROMATOGRAPHY A,1216(11),2098-2103.
MLA Yang, Li,et al."Rapid quantification of iridoid glycosides analogues in the formulated Chinese medicine Longdan Xiegan Decoction using high-performance liquid chromatography coupled with mass spectromentry".JOURNAL OF CHROMATOGRAPHY A 1216.11(2009):2098-2103.
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