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题名: Simply and reliably integrating micro heaters/sensors in a monolithic PCR-CE microfluidic genetic analysis system
作者: Zhong, Runtao1, 2;  Pan, Xiaoyan1, 2;  Jiang, Lei1;  Dai, Zhongpeng1;  Qin, Jianhua1;  Lin, Bingcheng1
通讯作者: 秦建华 ;  林炳承
关键词: Genetic analysis ;  Integrated microsystem ;  Microfabricated heater ;  PCR-CE microchip ;  Sensor
刊名: ELECTROPHORESIS
发表日期: 2009-04-01
DOI: 10.1002/elps.200800491
卷: 30, 期:8, 页:1297-1305
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel fabrication process was presented to construct a monolithic integrated PCR-CE microfluidic DNA analysis system as a step toward building a total genetic analysis microsystem. Microfabricated Titanium/Platinum (Ti/Pt) heaters and resistance temperature detectors (RTDs) were integrated on the backside of a bonded glass chip to provide good thermal transfer and precise temperature detection for the drilled PCR-wells. This heater/RTD integration procedure was simple and reliable, and the resulting metal layer can be easily renewed when the Ti/Pt layer was damaged in later use or novel heater/RTD design was desired. A straightforward "RTD-calibration" method was employed to optimize the chip-based thermal cycling conditions. This method was convenient and rapid, comparing with a conventional RTD-calibration/temperature adjustment method. The highest ramping rates of 14 degrees C/s for heating and 5 degrees C/s for cooling in a 3-mu L reaction volume allow 30 complete PCR cycles in about 33 min. After effectively passivating the PCR-well surface, successful lambda-phage DNA amplifications were achieved using a two- or three-temperature cycling protocol. The functionality and performance of the integrated microsystem were demonstrated by successful amplification and subsequent on-line separation/sizing of lambda-phage DNA. A rapid assay for Hepatitis B virus, one of the major human pathogens, was performed in less than 45 min, demonstrating that the developed PCR-CE microsystem was capable of performing automatic and high-speed genetic analysis.
关键词[WOS]: POLYMERASE-CHAIN-REACTION ;  CAPILLARY ELECTROPHORESIS MICROSYSTEM ;  DNA ANALYSIS ;  AMPLIFICATION ;  CHIP ;  DEVICE ;  SEPARATION ;  MICRODEVICE ;  EXTRACTION ;  SAMPLES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000265968300005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102057
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Zhong, Runtao,Pan, Xiaoyan,Jiang, Lei,et al. Simply and reliably integrating micro heaters/sensors in a monolithic PCR-CE microfluidic genetic analysis system[J]. ELECTROPHORESIS,2009,30(8):1297-1305.
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