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题名: Integrated microfluidic immunoassay for the rapid determination of clenbuterol
作者: Kong, Jing1, 2;  Jiang, Lei1;  Su, Xiaoou3;  Qin, Jianhua1;  Du, Yuguang1;  Lin, Bingcheng1
通讯作者: 秦建华 ;  林炳承 ;  杜昱光
刊名: LAB ON A CHIP
发表日期: 2009
DOI: 10.1039/b818430e
卷: 9, 期:11, 页:1541-1547
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805,1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: An integrated microfluidic immunoassay system was established for high throughput analysis of clenbuterol. This system consisted of an integrated microchip and a linear confocal laser induced fluorescence (LIF) scanner. The microchip was composed of three layers: a fluidic channel layer, a PDMS membrane layer and a pneumatic control layer. The multi-layer chip was integrated with 36 pneumatic micro-valves and multiple micro-pumps to realize the flexible reagent delivery, facilitating the automatic assays with less consumption of samples and reduced analysis time. The homemade LIF scanner was able to simultaneously detect multi-channels and provide the potential capability of high throughput assays. The performance of the system was demonstrated by the determination of clenbuterol, one of the most widely used beta-agonists. Under the optimal conditions, the linear range and the limit of detection of clenbuterol were 0 similar to 5.0 ng mL(-1) and 0.088 ng mL(-1), respectively. The recovery rates determined with pig urine samples of 1.0 ng mL(-1) and 2.0 ng mL(-1) were 98.74% and 102.51% (n = 3), respectively. The total detection time was less than 30 min. The system had the potential application for rapid detection of multiple beta-agonists in clinical, pharmaceutical and chemical analyses.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TANDEM MASS-SPECTROMETRY ;  CAPILLARY-ELECTROPHORESIS ;  PHARMACEUTICAL FORMULATIONS ;  CHEMILUMINESCENCE DETECTION ;  HUMAN URINE ;  HUMAN SERUM ;  GAS ;  RESIDUES ;  TISSUES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000266269400009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102065
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agriprod, Beijing 100081, Peoples R China

Recommended Citation:
Kong, Jing,Jiang, Lei,Su, Xiaoou,et al. Integrated microfluidic immunoassay for the rapid determination of clenbuterol[J]. LAB ON A CHIP,2009,9(11):1541-1547.
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