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题名: Microvalves actuated sandwich immunoassay on an integrated microfluidic system
作者: Gao, Xinghua1;  Jiang, Lei1;  Su, Xiaoou2;  Qin, Jianhua1;  Lin, Bingcheng1
通讯作者: 秦建华 ;  林炳承
关键词: Human immunoglobulin G ;  Immunoassay ;  Integration ;  Microfluidics ;  Microvalve
刊名: ELECTROPHORESIS
发表日期: 2009-07-01
DOI: 10.1002/elps.200800818
卷: 30, 期:14, 页:2481-2487
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this study, we developed an integrated and automatic microfluidic immunoassay system, which comprised a multi-layer immunoassay microfluidic chip and a rotary confocal LIF scanner. The normally closed elastomeric microvalves were integrated on the multi-layer microfluidic chip to realize flexible reagents delivery for the rapid immunoassay. The various operations of immunoassay including coating, blocking and washing could be performed on a single chip with reduced reagents consumption and analysis time. The homemade rotary LIF scanner was able to detect the signals from the multichannels sequentially in a short period of time, enabling the potential capability for high-throughput immunoassay. The performance of this system was demonstrated by the sandwich immunoassay of human immunoglobulin G with a LOD < 10 ng/mL (S/N = 3). The linear range was from 500 ng/mL to 100 mu g/mL. This system has also been applied to the measurement of human immunoglobulin G from 15 real samples, demonstrating the potential of this platform for biomedical applications.
关键词[WOS]: LARGE-SCALE INTEGRATION ;  ON-CHIP ;  VALVES ;  SAMPLES ;  DEVICE ;  PUMPS ;  ASSAY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000269041500010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102069
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agriprod, Beijing, Peoples R China

Recommended Citation:
Gao, Xinghua,Jiang, Lei,Su, Xiaoou,et al. Microvalves actuated sandwich immunoassay on an integrated microfluidic system[J]. ELECTROPHORESIS,2009,30(14):2481-2487.
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