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题名: Development of micropump-actuated negative pressure pinched injection for parallel electrophoresis on array microfluidic chip
作者: Li, Bowei1, 2;  Jiang, Lei1;  Xie, Hua1, 2;  Gao, Yan1;  Qin, Jianhua1;  Lin, Bingcheng1
通讯作者: 秦建华 ;  林炳承
关键词: Array ;  Injection ;  Microfluidic chip ;  Microvalve
刊名: ELECTROPHORESIS
发表日期: 2009-09-01
DOI: 10.1002/elps.200900177
卷: 30, 期:17, 页:3053-3057
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A micropump-actuated negative pressure pinched injection method is developed for parallel electrophoresis on a multi-channel LIF detection system. The system has a home-made device that could individually control 16-port solenoid valves and a high-voltage power supply. The laser beam is excitated and distributes to the array separation channels for detection. The hybrid Glass-PDMS microfluidic chip comprises two common reservoirs, four separation channels coupled to their respective pneumatic micropumps and two reference channels. Due to use of pressure as a driving force, the proposed method has no sample bias effect for separation. There is only one high-voltage supply needed for separation without relying on the number of channels, which is significant for high-throughput analysis, and the time for sample loading is shortened to 1 s. In addition, the integrated micropumps can provide the versatile interface for coupling with other function units to satisfy the complicated demands. The performance is verified by separation of DNA marker and Hepatitis B virus DNA samples. And this method is also expected to show the potential throughput for the DNA analysis in the field of disease diagnosis.
关键词[WOS]: THROUGHPUT GENETIC-ANALYSIS ;  GATED SAMPLE INTRODUCTION ;  CAPILLARY-ELECTROPHORESIS ;  MICROCHIP ELECTROPHORESIS ;  MULTICHANNEL MICROCHIP ;  PERFORMANCE ;  DEVICES ;  SYSTEM ;  IMMUNOASSAY ;  SEPARATIONS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000270015500012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102071
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China

Recommended Citation:
Li, Bowei,Jiang, Lei,Xie, Hua,et al. Development of micropump-actuated negative pressure pinched injection for parallel electrophoresis on array microfluidic chip[J]. ELECTROPHORESIS,2009,30(17):3053-3057.
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