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题名: Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device
作者: Li, Chunyu1, 2;  Dong, Xiuling3;  Qin, Jianhua1;  Lin, Bingcheng1
通讯作者: 秦建华 ;  林炳承
关键词: Reciprocating flow ;  DNA hybridization ;  Microfluidics ;  Centrifugal force ;  Capillary action ;  Dengue virus
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2009-04-27
DOI: 10.1016/j.aca.2009.03.026
卷: 640, 期:1-2, 页:93-99
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: We present a compact disk (CD) microfluidic device capable of generating the reciprocating flow of DNA samples within the microchannels and demonstrate its application in rapid DNA hybridization assay with nanoliter-volume samples. This device consists of a polydimethylsiloxane (PDMS) CD slab containing twelve DNA hybridization functional units and a glass substrate with immobilized DNA probe array. A reciprocating flow is produced with simple rotation-pause operation of the CD device. When spinning the CD device, centrifugal force drives the sample solution to now through the hybridization channel into the temporary collection reservoir. When stopping the rotation of the CD device, capillary action pulls the sample solution to reversely flow back into the hybridization channel because of hydrophilic surface of the hybridization channel. We used Dengue virus gene sequence (18mer) as a model to demonstrate that the reciprocating flow of DNA samples significantly enhanced the mass transfer rate in the hybridization reaction. reducing the hybridization time to 90 s. Moreover, only nanoliter-volume DNA samples were required per assay unit. We also compared the performance between the reciprocating-flow hybridization and the flow-through hybridization using the same sample concentrations. A fluorescence intensity was observed to increase up to threefold in the reciprocating-flow hybridization compared to the How-through hybridization with the same hybridization time (90 s) and sample volumes (350 nL). This CD microfluidic device has the potential for automated, rapid and multiple DNA-based diagnostics. (c) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: MICROARRAY HYBRIDIZATION ;  POLY(DIMETHYLSILOXANE) ;  OLIGONUCLEOTIDE ;  MICROCHIPS ;  CHIP ;  CD ;  MUTATIONS ;  SYSTEMS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000265763500014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102113
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Dalian Third Municipal Hosp, Dalian 116033, Peoples R China

Recommended Citation:
Li, Chunyu,Dong, Xiuling,Qin, Jianhua,et al. Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device[J]. ANALYTICA CHIMICA ACTA,2009,640(1-2):93-99.
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