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题名: Changes of the plasma metabolome during an oral glucose tolerance test: is there more than glucose to look at?
作者: Zhao, Xinjie2;  Peter, Andreas1;  Fritsche, Jens4;  Elcnerova, Michaela5;  Fritsche, Andreas3;  Haering, Hans-Ulrich3;  Schleicher, Erwin D.1;  Xu, Guowang2;  Lehmann, Rainer1
通讯作者: Rainer Lehmann
关键词: metabolomics ;  metabonomics ;  oral glucose tolerance test ;  bile acids ;  fatty acids ;  palmitoleate ;  acylcarnitines ;  lysophosphatidylcholines
刊名: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
发表日期: 2009-02-01
DOI: 10.1152/ajpendo.90748.2008
卷: 296, 期:2, 页:E384-E393
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Endocrinology & Metabolism ;  Physiology
研究领域[WOS]: Endocrinology & Metabolism ;  Physiology
英文摘要: Zhao X, Peter A, Fritsche J, Elcnerova M, Fritsche A, Haring HU, Schleicher ED, Xu G, Lehmann R. Changes of the plasma metabolome during an oral glucose tolerance test: is there more than glucose to look at? Am J Physiol Endocrinol Metab 296: E384-E393, 2009. First published December 9, 2008; doi: 10.1152/ajpendo.90748.2008.-The oral glucose tolerance test (oGTT) is a common tool to provoke a metabolic challenge for scientific purposes, as well as for diagnostic reasons, to monitor the kinetics of glucose and insulin. Here, we aimed to follow the variety of physiological changes of the whole metabolic pattern in plasma during an oGTT in healthy subjects in a nontargeted reversed-phase ultra performance liquid chromatography coupled to electrospray ionization quadrupole time of flight mass spectrometric metabolomics approach. We detected 11,500 metabolite ion masses/individual. Applying multivariate data analysis, four major groups of metabolites have been detected as the most discriminating oGTT biomarkers: free fatty acids (FFA), acylcarnitines, bile acids, and lysophosphatidylcholines. We found in detail 1) a strong decrease of all saturated and monounsaturated FFA studied during the oGTT; 2) a significant faster decline of palmitoleate (C16:1) and oleate (C18:1) FFA levels than their saturated counterparts; 3) a strong relative increase of polyunsaturated fatty acids in the fatty acid pattern at 120 min; and 4) a clear decrease in plasma C10:0, C12:0, and C14:1 acylcarnitine levels. These data reflect the switch from beta-oxidation to glycolysis and fat storage during the oGTT. Moreover, the bile acids glycocholic acid, glycochenodeoxycholic acid, and glycodeoxycholic acid were highly discriminative, showing a biphasic kinetic with a maximum of a 4.5- to 6- fold increase at 30 min after glucose ingestion, a significant decrease over the next 60 min followed by an increase until the end of the oGTT. Lysophosphatidylcholines were also increased significantly. The findings of our metabolomics study reveal detailed insights in the complex physiological regulation of the metabolism during an oGTT offering novel perspectives of this widely used procedure.
关键词[WOS]: FARNESOID-X-RECEPTOR ;  TYPE-2 DIABETES-MELLITUS ;  ARTERY ENDOTHELIAL-CELLS ;  FACTOR-KAPPA-B ;  BILE-ACIDS ;  INSULIN SENSITIVITY ;  FATTY-ACIDS ;  MASS-SPECTROMETRY ;  DATA SETS ;  LYSOPHOSPHATIDYLCHOLINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000262783100020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102129
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Hosp Tuebingen, Div Clin Chem & Pathobiochem, Cent Lab, D-72076 Tubingen, Germany
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
3.Univ Hosp Tuebingen, Dept Internal Med 4, D-72076 Tubingen, Germany
4.Immat Biotechnol, Tubingen, Germany
5.Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Pardubice, Czech Republic

Recommended Citation:
Zhao, Xinjie,Peter, Andreas,Fritsche, Jens,et al. Changes of the plasma metabolome during an oral glucose tolerance test: is there more than glucose to look at?[J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM,2009,296(2):E384-E393.
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