DICP OpenIR
Changes of the plasma metabolome during an oral glucose tolerance test: is there more than glucose to look at?
Zhao, Xinjie2; Peter, Andreas1; Fritsche, Jens4; Elcnerova, Michaela5; Fritsche, Andreas3; Haering, Hans-Ulrich3; Schleicher, Erwin D.1; Xu, Guowang2; Lehmann, Rainer1; Rainer Lehmann
关键词Metabolomics Metabonomics Oral Glucose Tolerance Test Bile Acids Fatty Acids Palmitoleate Acylcarnitines Lysophosphatidylcholines
刊名AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
2009-02-01
DOI10.1152/ajpendo.90748.2008
296期:2页:E384-E393
收录类别SCI
文章类型Article
部门归属18
项目归属1808
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Endocrinology & Metabolism ; Physiology
研究领域[WOS]Endocrinology & Metabolism ; Physiology
关键词[WOS]FARNESOID-X-RECEPTOR ; TYPE-2 DIABETES-MELLITUS ; ARTERY ENDOTHELIAL-CELLS ; FACTOR-KAPPA-B ; BILE-ACIDS ; INSULIN SENSITIVITY ; FATTY-ACIDS ; MASS-SPECTROMETRY ; DATA SETS ; LYSOPHOSPHATIDYLCHOLINE
英文摘要Zhao X, Peter A, Fritsche J, Elcnerova M, Fritsche A, Haring HU, Schleicher ED, Xu G, Lehmann R. Changes of the plasma metabolome during an oral glucose tolerance test: is there more than glucose to look at? Am J Physiol Endocrinol Metab 296: E384-E393, 2009. First published December 9, 2008; doi: 10.1152/ajpendo.90748.2008.-The oral glucose tolerance test (oGTT) is a common tool to provoke a metabolic challenge for scientific purposes, as well as for diagnostic reasons, to monitor the kinetics of glucose and insulin. Here, we aimed to follow the variety of physiological changes of the whole metabolic pattern in plasma during an oGTT in healthy subjects in a nontargeted reversed-phase ultra performance liquid chromatography coupled to electrospray ionization quadrupole time of flight mass spectrometric metabolomics approach. We detected 11,500 metabolite ion masses/individual. Applying multivariate data analysis, four major groups of metabolites have been detected as the most discriminating oGTT biomarkers: free fatty acids (FFA), acylcarnitines, bile acids, and lysophosphatidylcholines. We found in detail 1) a strong decrease of all saturated and monounsaturated FFA studied during the oGTT; 2) a significant faster decline of palmitoleate (C16:1) and oleate (C18:1) FFA levels than their saturated counterparts; 3) a strong relative increase of polyunsaturated fatty acids in the fatty acid pattern at 120 min; and 4) a clear decrease in plasma C10:0, C12:0, and C14:1 acylcarnitine levels. These data reflect the switch from beta-oxidation to glycolysis and fat storage during the oGTT. Moreover, the bile acids glycocholic acid, glycochenodeoxycholic acid, and glycodeoxycholic acid were highly discriminative, showing a biphasic kinetic with a maximum of a 4.5- to 6- fold increase at 30 min after glucose ingestion, a significant decrease over the next 60 min followed by an increase until the end of the oGTT. Lysophosphatidylcholines were also increased significantly. The findings of our metabolomics study reveal detailed insights in the complex physiological regulation of the metabolism during an oGTT offering novel perspectives of this widely used procedure.
语种英语
原文出处查看原文
WOS记录号WOS:000262783100020
引用统计
被引频次:112[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102129
专题中国科学院大连化学物理研究所
通讯作者Rainer Lehmann
作者单位1.Univ Hosp Tuebingen, Div Clin Chem & Pathobiochem, Cent Lab, D-72076 Tubingen, Germany
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
3.Univ Hosp Tuebingen, Dept Internal Med 4, D-72076 Tubingen, Germany
4.Immat Biotechnol, Tubingen, Germany
5.Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Pardubice, Czech Republic
推荐引用方式
GB/T 7714
Zhao, Xinjie,Peter, Andreas,Fritsche, Jens,et al. Changes of the plasma metabolome during an oral glucose tolerance test: is there more than glucose to look at?[J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM,2009,296(2):E384-E393.
APA Zhao, Xinjie.,Peter, Andreas.,Fritsche, Jens.,Elcnerova, Michaela.,Fritsche, Andreas.,...&Rainer Lehmann.(2009).Changes of the plasma metabolome during an oral glucose tolerance test: is there more than glucose to look at?.AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM,296(2),E384-E393.
MLA Zhao, Xinjie,et al."Changes of the plasma metabolome during an oral glucose tolerance test: is there more than glucose to look at?".AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 296.2(2009):E384-E393.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Xinjie]的文章
[Peter, Andreas]的文章
[Fritsche, Jens]的文章
百度学术
百度学术中相似的文章
[Zhao, Xinjie]的文章
[Peter, Andreas]的文章
[Fritsche, Jens]的文章
必应学术
必应学术中相似的文章
[Zhao, Xinjie]的文章
[Peter, Andreas]的文章
[Fritsche, Jens]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。