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题名: Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope
作者: Lv, Guojun1, 2;  Sun, Zhijie1, 2;  Li, Shuangyue1, 2;  Yu, Weiting1, 2;  Xie, Yubing1;  Zhang, Ying1, 2;  Xie, Hongguo1, 2;  Li, Xiaoxia1, 2;  Wang, Wei1;  Ma, Xiaojun1
通讯作者: 马小军
关键词: cell encapsulation ;  alginate ;  membrane permeability ;  protein fouling ;  confocal laser scanning microscope (CLSM)
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2009-09-01
DOI: 10.1002/jbm.a.32115
卷: 90A, 期:3, 页:773-783
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: Microencapsulation of recombinant cells is a novel means for gene therapy. However, one of the major concerns is the relationship between the permeability of microcapsule and cell growth. Many studies have focused on the permeability of empty microcapsule, but little is known about the effect of the cell growth on the permeability of a cell-contained microcapsule. A combination of fluorescence labeled protein and confocal laser scanning microscope (CLSM) provides the information about the permeability changes during the Cell growth. A decrease of membrane permeability was detected oil the 14th day. Meanwhile, membrane surface protein fouling was also investigated. A significant increase of membrane surface protein content was detected on the 21st day. In order to study the effect of the permeability changes on the cell viability, the membrane of cell-contained microcapsules with different permeability Was Set up by incubating gel beads in poly-L-lysine for 5 and 30 min, respectively, to mimic the bovine serum albumin cutoff, and a retard of cell growth was found in 7 days' Culture. These results showed that the protein fouling of the microcapsule membrane caused by the cell growth may be in important factor to influence cell viability. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 96A: 773-783, 2009
关键词[WOS]: MICROFILTRATION MEMBRANES ;  ALGINATE MICROCAPSULES ;  POLY(ETHYLENE OXIDE) ;  DIFFUSION ;  MODEL ;  MICROENCAPSULATION ;  MACROMOLECULES ;  RELEASE ;  GELS ;  TRANSPLANTATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000268860100017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102147
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Lv, Guojun,Sun, Zhijie,Li, Shuangyue,et al. Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2009,90A(3):773-783.
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