DICP OpenIR
Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope
Lv, Guojun1,2; Sun, Zhijie1,2; Li, Shuangyue1,2; Yu, Weiting1,2; Xie, Yubing1; Zhang, Ying1,2; Xie, Hongguo1,2; Li, Xiaoxia1,2; Wang, Wei1; Ma, Xiaojun1; Ma XJ(马小军)
关键词Cell Encapsulation Alginate Membrane Permeability Protein Fouling Confocal Laser Scanning Microscope (Clsm)
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2009-09-01
DOI10.1002/jbm.a.32115
90A期:3页:773-783
收录类别SCI
文章类型Article
部门归属18
项目归属1802
产权排名1;1
WOS标题词Science & Technology ; Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]MICROFILTRATION MEMBRANES ; ALGINATE MICROCAPSULES ; POLY(ETHYLENE OXIDE) ; DIFFUSION ; MODEL ; MICROENCAPSULATION ; MACROMOLECULES ; RELEASE ; GELS ; TRANSPLANTATION
英文摘要Microencapsulation of recombinant cells is a novel means for gene therapy. However, one of the major concerns is the relationship between the permeability of microcapsule and cell growth. Many studies have focused on the permeability of empty microcapsule, but little is known about the effect of the cell growth on the permeability of a cell-contained microcapsule. A combination of fluorescence labeled protein and confocal laser scanning microscope (CLSM) provides the information about the permeability changes during the Cell growth. A decrease of membrane permeability was detected oil the 14th day. Meanwhile, membrane surface protein fouling was also investigated. A significant increase of membrane surface protein content was detected on the 21st day. In order to study the effect of the permeability changes on the cell viability, the membrane of cell-contained microcapsules with different permeability Was Set up by incubating gel beads in poly-L-lysine for 5 and 30 min, respectively, to mimic the bovine serum albumin cutoff, and a retard of cell growth was found in 7 days' Culture. These results showed that the protein fouling of the microcapsule membrane caused by the cell growth may be in important factor to influence cell viability. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 96A: 773-783, 2009
语种英语
原文出处查看原文
WOS记录号WOS:000268860100017
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102147
专题中国科学院大连化学物理研究所
通讯作者Ma XJ(马小军)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Lv, Guojun,Sun, Zhijie,Li, Shuangyue,et al. Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2009,90A(3):773-783.
APA Lv, Guojun.,Sun, Zhijie.,Li, Shuangyue.,Yu, Weiting.,Xie, Yubing.,...&马小军.(2009).Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,90A(3),773-783.
MLA Lv, Guojun,et al."Permeability changes of the cell-contained microcapsules visualized by confocal laser scanning microscope".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 90A.3(2009):773-783.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lv, Guojun]的文章
[Sun, Zhijie]的文章
[Li, Shuangyue]的文章
百度学术
百度学术中相似的文章
[Lv, Guojun]的文章
[Sun, Zhijie]的文章
[Li, Shuangyue]的文章
必应学术
必应学术中相似的文章
[Lv, Guojun]的文章
[Sun, Zhijie]的文章
[Li, Shuangyue]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。