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题名: Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry
作者: Zhang, Fengxia2, 3;  Jia, Zhenhua1;  Gao, Peng3;  Kong, Hongwei3;  Li, Xiang3;  Chen, Jing3;  Yang, Qin3;  Yin, Peiyuan3;  Wang, Jiangshan3;  Lu, Xin3;  Li, Famei2;  Wu, Yiling1;  Xu, Guowang2, 3
通讯作者: 许国旺
关键词: Atherosclerosis ;  Metabonomics ;  UFLC/MS-IT-TOF ;  Plasma ;  Urine ;  Rats
刊名: TALANTA
发表日期: 2009-08-15
DOI: 10.1016/j.talanta.2009.05.010
卷: 79, 期:3, 页:836-844
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An ultra fast liquid chromatography coupled with IT-TOF mass spectrometry (UFLC/MS-IT-TOF) metabonomic approach was employed to study the plasma and urine metabolic profiling of atherosclerosis rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the atherosclerosis and the control groups. Potential biomarkers were screened by using S-plot and were identified by the accurate mass and MS(n) fragments information obtained from UFLC/MS-IT-TOF analysis. 12 metabolites in rat plasma and 8 metabolites in urine were identified as potential biomarkers. Concentrations of leucine, phenylalanine, tryptophan, acetylcarnitine, butyrylcarnitine, propionylcarnitine and spermine in plasma and 3-O-methyl-dopa, ethyl, N2-acetyl-L-argininate, leucylproline, glucuronate, t6A N(6)-(N-threonylcarbonyl)-adenosine and methyl-hippuric acid in urine decreased in atherosclerosis rats. Ursodeoxycholic acid, chenodeoxycholic acid, LPC (06:0), LPC (C18:0) and LPC (C18:1) in plasma and hippuric acid in urine were in higher levels in atherosclerosis rats. The alterated metabolites demonstrated abnormal metabolism of phenylalanine, tryptophan, bile acids and amino acids. This research proved that metabonomics is a promising tool for disease research. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: MULTIVARIATE STATISTICAL-ANALYSIS ;  MOUSE IGA NEPHROPATHY ;  POTENTIAL BIOMARKERS ;  PATTERN-RECOGNITION ;  SYSTEMS BIOLOGY ;  BILE-ACIDS ;  UPLC-MS ;  TOF-MS ;  LYSOPHOSPHATIDYLCHOLINE ;  IDENTIFICATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000268346800042
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102157
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Integrat Tradit & Western Med Res Acad Hebei Prov, Shijiazhuang 050035, Hebei Province, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Pharm, Dept Analyt Chem, Shenyang 110016, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Fengxia,Jia, Zhenhua,Gao, Peng,et al. Metabonomics study of atherosclerosis rats by ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry[J]. TALANTA,2009,79(3):836-844.
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