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Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports
Sun, J.1; Zhou, Y.2; Yuan, Z.3; Xu, G.1; Xu GW(许国旺)
Source PublicationAPPLIED BIOCHEMISTRY AND MICROBIOLOGY
2009-09-01
DOI10.1134/S0003683809050032
Volume45Issue:5Pages:478-483
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS KeywordMULTIPOINT COVALENT ATTACHMENT ; SALT-INDUCED IMMOBILIZATION ; ACTIVATED SUPPORTS ; GLUTARYL ACYLASE ; PROTEINS ; PERFORMANCE ; SEPABEADS ; ENZYMES ; TOOL
AbstractAlcaligenes faecalis penicillin G acylase has several desired features over other penicillin G acylases and its use in industry requires immobilization. In this work, two novel supports ZH-EP (epoxy type) and ZH-HA (epoxy-amino type) were used to immobilize Alcaligenes faecalis penicillin G acylase (AfPGA) with Eupergit C as reference. The saturation of immobilized protein on ZH-EP (269 mg/g, 116 h) and ZH-HA (296 mg/g, 15 h) was obtained more rapidly than Eupergit C (197 mg/g, 260 h). And the activity of immobilized AfPGA on ZH-EP (520 U/g) and ZH-HA (2200 U/g) was higher than that on Eupergit C (310 U/g). The propertics of three immobilized enzymes were compared and no obvious difference was observed, which indicated that ZH-EP and ZH-HA were promised in industry.
Language英语
URL查看原文
WOS IDWOS:000269885800003
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102167
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Life Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
Recommended Citation
GB/T 7714
Sun, J.,Zhou, Y.,Yuan, Z.,et al. Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports[J]. APPLIED BIOCHEMISTRY AND MICROBIOLOGY,2009,45(5):478-483.
APA Sun, J.,Zhou, Y.,Yuan, Z.,Xu, G.,&许国旺.(2009).Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports.APPLIED BIOCHEMISTRY AND MICROBIOLOGY,45(5),478-483.
MLA Sun, J.,et al."Immobilization of Alcaligenes faecalis Penicillin G Acylase on Epoxy-Type Supports".APPLIED BIOCHEMISTRY AND MICROBIOLOGY 45.5(2009):478-483.
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