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题名: Columns switch recycling size exclusion chromatography for high resolution protein separation
作者: Yuan, Huiming1, 2;  Zhang, Lihua1;  Zhang, Weibing3;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Columns switch recycling size exclusion chromatography ;  Multidimensional HPLC ;  Protein separation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-10-16
DOI: 10.1016/j.chroma.2009.08.065
卷: 1216, 期:42, 页:7024-7032
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Columns switch recycling size exclusion chromatography (csrSEC) was proposed to achieve high resolution protein separation with good biocompatibility. Proteins were firstly separated by two serially coupled SEC columns, and fractions were in sequence switched back to the first column by two-position valves for further separation in terms of close-loop recycling until satisfactory resolution was achieved. Compared to SEC, the separation window was broadened by increasing column length via cycling without further increase on back pressure. Compared to recycling SEC (rSEC), the overtaking of later eluted components by early eluted ones after several cycles could be avoided for complex sample analysis, by parking fractions in the second SEC column before transferred in turn back to the first one for cycling ordinally. In our experiments, the baseline separation of five proteins with molecular weight ranging from 10 kDa to 80 kDa was achieved by csrSEC. Furthermore, a multidimensional csrSEC-RPLC platform was constructed, and peak capacity up to 3600 was obtained for protein separation. All these results demonstrated that csrSEC is a promising protein separation mode with good biocompatibility, broadened separation window and improved resolution. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY LIQUID-CHROMATOGRAPHY ;  2-DIMENSIONAL HPLC ;  PEAK-CAPACITY ;  PURIFICATION ;  MODULATION ;  PHASE ;  LC
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000270750900007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102169
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.E China Univ Sci & Technol, Sch Chem & Mol Engn, Dept Chem, Shanghai 200237, Peoples R China

Recommended Citation:
Yuan, Huiming,Zhang, Lihua,Zhang, Weibing,et al. Columns switch recycling size exclusion chromatography for high resolution protein separation[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(42):7024-7032.
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