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Columns switch recycling size exclusion chromatography for high resolution protein separation
Yuan, Huiming1,2; Zhang, Lihua1; Zhang, Weibing3; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordColumns Switch Recycling Size Exclusion Chromatography Multidimensional Hplc Protein Separation
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2009-10-16
DOI10.1016/j.chroma.2009.08.065
Volume1216Issue:42Pages:7024-7032
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCAPILLARY LIQUID-CHROMATOGRAPHY ; 2-DIMENSIONAL HPLC ; PEAK-CAPACITY ; PURIFICATION ; MODULATION ; PHASE ; LC
AbstractColumns switch recycling size exclusion chromatography (csrSEC) was proposed to achieve high resolution protein separation with good biocompatibility. Proteins were firstly separated by two serially coupled SEC columns, and fractions were in sequence switched back to the first column by two-position valves for further separation in terms of close-loop recycling until satisfactory resolution was achieved. Compared to SEC, the separation window was broadened by increasing column length via cycling without further increase on back pressure. Compared to recycling SEC (rSEC), the overtaking of later eluted components by early eluted ones after several cycles could be avoided for complex sample analysis, by parking fractions in the second SEC column before transferred in turn back to the first one for cycling ordinally. In our experiments, the baseline separation of five proteins with molecular weight ranging from 10 kDa to 80 kDa was achieved by csrSEC. Furthermore, a multidimensional csrSEC-RPLC platform was constructed, and peak capacity up to 3600 was obtained for protein separation. All these results demonstrated that csrSEC is a promising protein separation mode with good biocompatibility, broadened separation window and improved resolution. (C) 2009 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000270750900007
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102169
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.E China Univ Sci & Technol, Sch Chem & Mol Engn, Dept Chem, Shanghai 200237, Peoples R China
Recommended Citation
GB/T 7714
Yuan, Huiming,Zhang, Lihua,Zhang, Weibing,et al. Columns switch recycling size exclusion chromatography for high resolution protein separation[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(42):7024-7032.
APA Yuan, Huiming,Zhang, Lihua,Zhang, Weibing,Liang, Zhen,Zhang, Yukui,&张丽华.(2009).Columns switch recycling size exclusion chromatography for high resolution protein separation.JOURNAL OF CHROMATOGRAPHY A,1216(42),7024-7032.
MLA Yuan, Huiming,et al."Columns switch recycling size exclusion chromatography for high resolution protein separation".JOURNAL OF CHROMATOGRAPHY A 1216.42(2009):7024-7032.
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