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题名: Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations
作者: Chen, Jing1;  Wang, Wenzhao1;  Lv, Shen2;  Yin, Peiyuan1;  Zhao, Xinjie1;  Lu, Xin1;  Zhang, Fengxia1;  Xu, Guowang1
通讯作者: 许国旺
关键词: Metabonomics ;  Metabolomics ;  Hydrophilic interaction chromatography ;  Ultra performance liquid chromatography-mass spectrometry ;  Liver cancer ;  Biomarker
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2009-09-14
DOI: 10.1016/j.aca.2009.03.039
卷: 650, 期:1, 页:3-9
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: In this study, urinary metabolites from liver cancer patients and healthy volunteers were studied by a metabonomic method based on ultra performance liquid chromatography coupled to mass spectrometry. Both hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) were used to separate the urinary metabolites. Principle component analysis (PCA) and partial least squares to latent structure-discriminant analysis (PLS-DA) models were built to separate the healthy volunteers from the liver cancer patients and to find compounds that are expressed in significantly different amounts between the two populations. 21 metabolite ions were considered as potential biomarkers according to the Variable importance in the Project (VIP) value and S-plot. Compared with RPLC, a more sensitive and stable response can be recorded in HILIC mode due to the high content of organic solvent used. Moreover, the liver cancer group and the healthy volunteers can be better separated based on the data from the HILIC separation, which indicates that HILIC is suitable for urinary metabonomic analysis. In HILIC mode, several polar compounds related to arginine and proline metabolism, alanine and aspartate metabolism, lysine degradation, nicotinate and nicotinamide metabolism were found to be significantly changed in the concentrations of the two different populations: healthy and cancer. In contrast, in RPLC mode, these changed compounds are related to fatty acids oxidation. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  PROLIDASE DEFICIENCY ;  RAT URINE ;  TOF-MS ;  IDENTIFICATION ;  METABOLOMICS ;  ELECTROSPRAY ;  DISCOVERY ;  COLUMN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000270003800002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102171
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China

Recommended Citation:
Chen, Jing,Wang, Wenzhao,Lv, Shen,et al. Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations[J]. ANALYTICA CHIMICA ACTA,2009,650(1):3-9.
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