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Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations
Chen, Jing1; Wang, Wenzhao1; Lv, Shen2; Yin, Peiyuan1; Zhao, Xinjie1; Lu, Xin1; Zhang, Fengxia1; Xu, Guowang1; Xu GW(许国旺)
KeywordMetabonomics Metabolomics Hydrophilic Interaction Chromatography Ultra Performance Liquid Chromatography-mass Spectrometry Liver Cancer Biomarker
Source PublicationANALYTICA CHIMICA ACTA
2009-09-14
DOI10.1016/j.aca.2009.03.039
Volume650Issue:1Pages:3-9
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHYDROPHILIC INTERACTION CHROMATOGRAPHY ; PROLIDASE DEFICIENCY ; RAT URINE ; TOF-MS ; IDENTIFICATION ; METABOLOMICS ; ELECTROSPRAY ; DISCOVERY ; COLUMN
AbstractIn this study, urinary metabolites from liver cancer patients and healthy volunteers were studied by a metabonomic method based on ultra performance liquid chromatography coupled to mass spectrometry. Both hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) were used to separate the urinary metabolites. Principle component analysis (PCA) and partial least squares to latent structure-discriminant analysis (PLS-DA) models were built to separate the healthy volunteers from the liver cancer patients and to find compounds that are expressed in significantly different amounts between the two populations. 21 metabolite ions were considered as potential biomarkers according to the Variable importance in the Project (VIP) value and S-plot. Compared with RPLC, a more sensitive and stable response can be recorded in HILIC mode due to the high content of organic solvent used. Moreover, the liver cancer group and the healthy volunteers can be better separated based on the data from the HILIC separation, which indicates that HILIC is suitable for urinary metabonomic analysis. In HILIC mode, several polar compounds related to arginine and proline metabolism, alanine and aspartate metabolism, lysine degradation, nicotinate and nicotinamide metabolism were found to be significantly changed in the concentrations of the two different populations: healthy and cancer. In contrast, in RPLC mode, these changed compounds are related to fatty acids oxidation. (C) 2009 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000270003800002
Citation statistics
Cited Times:158[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102171
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Chen, Jing,Wang, Wenzhao,Lv, Shen,et al. Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations[J]. ANALYTICA CHIMICA ACTA,2009,650(1):3-9.
APA Chen, Jing.,Wang, Wenzhao.,Lv, Shen.,Yin, Peiyuan.,Zhao, Xinjie.,...&许国旺.(2009).Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations.ANALYTICA CHIMICA ACTA,650(1),3-9.
MLA Chen, Jing,et al."Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations".ANALYTICA CHIMICA ACTA 650.1(2009):3-9.
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