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Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with external modification
Qi, Yanxia1; Wei, Junying2; Wang, Hua1; Zhang, Yangjun2; Xu, Jing1; Qian, Xiaohong2; Guan, Yafeng1; Guan YF(关亚风)
关键词Selective Extraction Low Molecular Weight Proteins External Modification Mesoporous Material Human Plasma
刊名TALANTA
2009-12-15
DOI10.1016/j.talanta.2009.07.050
80期:2页:703-709
收录类别SCI
文章类型Article
部门归属1
项目归属105
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]LOW-ABUNDANCE PEPTIDES ; BOVINE SERUM-ALBUMIN ; TOF MS ANALYSIS ; MCM-41 ; EXTRACTION ; PHASE ; PERFORMANCE ; ADSORPTION ; ENRICHMENT ; SAMPLES
英文摘要Selective extraction of low molecular weight (LMW) proteins and peptides from complex biological samples plays an important role in the discovery of useful biomarkers and signaling molecules. Various methods, such as solid-phase extraction (SPE), ultrafiltration, and size-exclusion chromatography have been developed for such extraction purpose. In this study, we present, to our knowledge, the first demonstration of alkyl-diol@SiO(2) mesoporous material MCM-41 (alkyl-diol group on the external surface of mesoporous material) for selective extraction of LMW proteins and peptides from complex biological samples. The adsorption kinetics of LMW proteins, the influence of pH on adsorption and the desorption recovery by different elution solvents were investigated by using standard proteins as model samples. It was demonstrated that the modification of alkyl-diol group on the external surface could efficiently decrease the adsorption of HMW protein and increase the desorption recovery of LMW protein. Furthermore, the mesoporous materials were applied to selectively extract LMW proteins and peptides (<10 kDa) from crude human plasma. And the modified MCM-41 material had much better extraction selectivity and efficiency for LMW proteins and peptides than unmodified one. (C) 2009 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000271701900044
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102207
专题中国科学院大连化学物理研究所
通讯作者Guan YF(关亚风)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat & Anal, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
2.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Proteom, Beijing 102206, Peoples R China
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Qi, Yanxia,Wei, Junying,Wang, Hua,et al. Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with external modification[J]. TALANTA,2009,80(2):703-709.
APA Qi, Yanxia.,Wei, Junying.,Wang, Hua.,Zhang, Yangjun.,Xu, Jing.,...&关亚风.(2009).Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with external modification.TALANTA,80(2),703-709.
MLA Qi, Yanxia,et al."Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with external modification".TALANTA 80.2(2009):703-709.
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