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题名: Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with external modification
作者: Qi, Yanxia1;  Wei, Junying2;  Wang, Hua1;  Zhang, Yangjun2;  Xu, Jing1;  Qian, Xiaohong2;  Guan, Yafeng1
通讯作者: 关亚风
关键词: Selective extraction ;  Low molecular weight proteins ;  External modification ;  Mesoporous material ;  Human plasma
刊名: TALANTA
发表日期: 2009-12-15
DOI: 10.1016/j.talanta.2009.07.050
卷: 80, 期:2, 页:703-709
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Selective extraction of low molecular weight (LMW) proteins and peptides from complex biological samples plays an important role in the discovery of useful biomarkers and signaling molecules. Various methods, such as solid-phase extraction (SPE), ultrafiltration, and size-exclusion chromatography have been developed for such extraction purpose. In this study, we present, to our knowledge, the first demonstration of alkyl-diol@SiO(2) mesoporous material MCM-41 (alkyl-diol group on the external surface of mesoporous material) for selective extraction of LMW proteins and peptides from complex biological samples. The adsorption kinetics of LMW proteins, the influence of pH on adsorption and the desorption recovery by different elution solvents were investigated by using standard proteins as model samples. It was demonstrated that the modification of alkyl-diol group on the external surface could efficiently decrease the adsorption of HMW protein and increase the desorption recovery of LMW protein. Furthermore, the mesoporous materials were applied to selectively extract LMW proteins and peptides (<10 kDa) from crude human plasma. And the modified MCM-41 material had much better extraction selectivity and efficiency for LMW proteins and peptides than unmodified one. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: LOW-ABUNDANCE PEPTIDES ;  BOVINE SERUM-ALBUMIN ;  TOF MS ANALYSIS ;  MCM-41 ;  EXTRACTION ;  PHASE ;  PERFORMANCE ;  ADSORPTION ;  ENRICHMENT ;  SAMPLES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000271701900044
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102207
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat & Anal, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
2.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Proteom, Beijing 102206, Peoples R China

Recommended Citation:
Qi, Yanxia,Wei, Junying,Wang, Hua,et al. Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with external modification[J]. TALANTA,2009,80(2):703-709.
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