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题名: Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles
作者: Song, Cuihong1;  Ye, Zhiqiang2;  Wang, Guilan2;  Jin, Dayong3;  Yuan, Jingli1, 2;  Guan, Yafeng1;  Piper, James3
通讯作者: 袁景利 ;  关亚风
关键词: Ruthenium complex ;  Luminescent nanoparticles ;  Bioimaging ;  Time-gated luminescence ;  Environmental pathogen
刊名: TALANTA
发表日期: 2009-06-30
DOI: 10.1016/j.talanta.2009.03.018
卷: 79, 期:1, 页:103-108
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Luminescent ruthenium(II) complex covalently bound silica nanoparticles have been prepared and used as a probe for time-gated luminescence bioimaging. The new nanoparticles were prepared by copolymerization of a luminescent Ru(II) complex tris(5-amino-1,10-phenanthroline)ruthenium(II) conjugated with 3-aminopropyl(triethoxy)si lane (APS-Ru conjugate), free (3 -aminopropyl)triethoxysilane (APS) and tetraethyl orthosilicate (TEOS) in a water-in-oil reverse microemulsion consisting of Triton X-100, n-octanol, cyclohexane and water in the presence of aqueous ammonia. Characterization by transmission electron microscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, 64 +/- 4 nm in diameter. Compared with the dye-doping nanoparticles, dye leakage of the new nanoparticles was remarkably decreased. In addition, it was found that the Ru(II) complex luminescence could be effectively enhanced with a longer luminescence lifetime (similar to 2.3 mu s) after forming the nanoparticles, which enables the nanoparticles to be suitable as a bioprobe for time-gated luminescence bioimaging applications. The nanoparticle-labeled streptavidin was prepared and successfully used for time-gated luminescence imaging detection of an environmental pathogen, Giardia lamblia, with high specificity and sensitivity. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: GIARDIA-LAMBLIA ;  BIOCONJUGATED NANOPARTICLES ;  FLUORESCENT NANOPARTICLES ;  EUROPIUM(III) COMPLEX ;  MICROSCOPY ;  UV ;  CRYPTOSPORIDIUM ;  EXCITATION ;  LIFETIMES ;  BACTERIA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000266187600018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102213
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dept Chem, Dalian 116012, Peoples R China
3.Macquarie Univ, MQ Photon Ctr, Fac Sci, Sydney, NSW 2109, Australia

Recommended Citation:
Song, Cuihong,Ye, Zhiqiang,Wang, Guilan,et al. Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles[J]. TALANTA,2009,79(1):103-108.
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