DICP OpenIR
Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles
Song, Cuihong1; Ye, Zhiqiang2; Wang, Guilan2; Jin, Dayong3; Yuan, Jingli1,2; Guan, Yafeng1; Piper, James3; Yuan JL(袁景利); Guan YF(关亚风)
KeywordRuthenium Complex Luminescent Nanoparticles Bioimaging Time-gated Luminescence Environmental Pathogen
Source PublicationTALANTA
2009-06-30
DOI10.1016/j.talanta.2009.03.018
Volume79Issue:1Pages:103-108
Indexed BySCI
SubtypeArticle
Department1
Funding Project105
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordGIARDIA-LAMBLIA ; BIOCONJUGATED NANOPARTICLES ; FLUORESCENT NANOPARTICLES ; EUROPIUM(III) COMPLEX ; MICROSCOPY ; UV ; CRYPTOSPORIDIUM ; EXCITATION ; LIFETIMES ; BACTERIA
AbstractLuminescent ruthenium(II) complex covalently bound silica nanoparticles have been prepared and used as a probe for time-gated luminescence bioimaging. The new nanoparticles were prepared by copolymerization of a luminescent Ru(II) complex tris(5-amino-1,10-phenanthroline)ruthenium(II) conjugated with 3-aminopropyl(triethoxy)si lane (APS-Ru conjugate), free (3 -aminopropyl)triethoxysilane (APS) and tetraethyl orthosilicate (TEOS) in a water-in-oil reverse microemulsion consisting of Triton X-100, n-octanol, cyclohexane and water in the presence of aqueous ammonia. Characterization by transmission electron microscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, 64 +/- 4 nm in diameter. Compared with the dye-doping nanoparticles, dye leakage of the new nanoparticles was remarkably decreased. In addition, it was found that the Ru(II) complex luminescence could be effectively enhanced with a longer luminescence lifetime (similar to 2.3 mu s) after forming the nanoparticles, which enables the nanoparticles to be suitable as a bioprobe for time-gated luminescence bioimaging applications. The nanoparticle-labeled streptavidin was prepared and successfully used for time-gated luminescence imaging detection of an environmental pathogen, Giardia lamblia, with high specificity and sensitivity. (C) 2009 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000266187600018
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102213
Collection中国科学院大连化学物理研究所
Corresponding AuthorYuan JL(袁景利); Guan YF(关亚风)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dept Chem, Dalian 116012, Peoples R China
3.Macquarie Univ, MQ Photon Ctr, Fac Sci, Sydney, NSW 2109, Australia
Recommended Citation
GB/T 7714
Song, Cuihong,Ye, Zhiqiang,Wang, Guilan,et al. Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles[J]. TALANTA,2009,79(1):103-108.
APA Song, Cuihong.,Ye, Zhiqiang.,Wang, Guilan.,Jin, Dayong.,Yuan, Jingli.,...&关亚风.(2009).Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles.TALANTA,79(1),103-108.
MLA Song, Cuihong,et al."Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles".TALANTA 79.1(2009):103-108.
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