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题名: Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition
作者: Li, Ning1, 2;  Zong, Min-Hua1;  Ma, Ding2
通讯作者: Minhua Zong ;  马丁
关键词: Thermomyces lanuginosus lipase ;  Nucleoside ;  Substrate recognition ;  Substrate engineering ;  Enzymatic acylation ;  Regioselectivity
刊名: JOURNAL OF BIOTECHNOLOGY
发表日期: 2009-03-25
DOI: 10.1016/j.jbiotec.2009.02.003
卷: 140, 期:3-4, 页:250-253
收录类别: SCI
文章类型: Article
部门归属: 5
项目归属: 502
产权排名: 2;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Substrate recognition of Thermomyces lanuginosus lipase in the acylation of nucleosides was revealed through rational substrate engineering for the first time. T lanuginosus lipase displayed higher catalytic activities and excellent 5'-regioselectivities (94 -> 99%) in the acylation of ribonucleosides 1f-1j as compared to those in the acylation of 2'-deoxynucleosides la-le. The higher reaction rates and excellent 5'-regioselectivities might derive from a favorable hydrogen bonding between the 2'-hydroxyl group of 1f-1j and phenolic hydroxyl group of Tyr21 present in the hydrophilic region of the lipase. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.
关键词[WOS]: PSEUDOMONAS-CEPACIA LIPASE ;  HUMICOLA-LANUGINOSA ;  ESTER DERIVATIVES ;  IONIC LIQUID ;  CLINICAL-USE ;  1-BETA-D-ARABINOFURANOSYLCYTOSINE ;  SYSTEMS ;  ENANTIOSELECTIVITY ;  CHEMOTHERAPY ;  FLOXURIDINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000265465500015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102329
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.S China Univ Technol, Lab Appl Biocatalysis, Guangzhou 510640, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China

Recommended Citation:
Li, Ning,Zong, Min-Hua,Ma, Ding. Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition[J]. JOURNAL OF BIOTECHNOLOGY,2009,140(3-4):250-253.
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