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Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition
Li, Ning1,2; Zong, Min-Hua1; Ma, Ding2; Minhua Zong; Ma D(马丁)
关键词Thermomyces Lanuginosus Lipase Nucleoside Substrate Recognition Substrate Engineering Enzymatic Acylation Regioselectivity
刊名JOURNAL OF BIOTECHNOLOGY
2009-03-25
DOI10.1016/j.jbiotec.2009.02.003
140期:3-4页:250-253
收录类别SCI
文章类型Article
部门归属5
项目归属502
产权排名2;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]PSEUDOMONAS-CEPACIA LIPASE ; HUMICOLA-LANUGINOSA ; ESTER DERIVATIVES ; IONIC LIQUID ; CLINICAL-USE ; 1-BETA-D-ARABINOFURANOSYLCYTOSINE ; SYSTEMS ; ENANTIOSELECTIVITY ; CHEMOTHERAPY ; FLOXURIDINE
英文摘要Substrate recognition of Thermomyces lanuginosus lipase in the acylation of nucleosides was revealed through rational substrate engineering for the first time. T lanuginosus lipase displayed higher catalytic activities and excellent 5'-regioselectivities (94 -> 99%) in the acylation of ribonucleosides 1f-1j as compared to those in the acylation of 2'-deoxynucleosides la-le. The higher reaction rates and excellent 5'-regioselectivities might derive from a favorable hydrogen bonding between the 2'-hydroxyl group of 1f-1j and phenolic hydroxyl group of Tyr21 present in the hydrophilic region of the lipase. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000265465500015
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102329
专题中国科学院大连化学物理研究所
通讯作者Minhua Zong; Ma D(马丁)
作者单位1.S China Univ Technol, Lab Appl Biocatalysis, Guangzhou 510640, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
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Li, Ning,Zong, Min-Hua,Ma, Ding,et al. Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition[J]. JOURNAL OF BIOTECHNOLOGY,2009,140(3-4):250-253.
APA Li, Ning,Zong, Min-Hua,Ma, Ding,Minhua Zong,&马丁.(2009).Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition.JOURNAL OF BIOTECHNOLOGY,140(3-4),250-253.
MLA Li, Ning,et al."Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition".JOURNAL OF BIOTECHNOLOGY 140.3-4(2009):250-253.
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