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Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition
Li, Ning1,2; Zong, Min-Hua1; Ma, Ding2; Minhua Zong; Ma D(马丁)
KeywordThermomyces Lanuginosus Lipase Nucleoside Substrate Recognition Substrate Engineering Enzymatic Acylation Regioselectivity
Source PublicationJOURNAL OF BIOTECHNOLOGY
2009-03-25
DOI10.1016/j.jbiotec.2009.02.003
Volume140Issue:3-4Pages:250-253
Indexed BySCI
SubtypeArticle
Department5
Funding Project502
Contribution Rank2;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordPSEUDOMONAS-CEPACIA LIPASE ; HUMICOLA-LANUGINOSA ; ESTER DERIVATIVES ; IONIC LIQUID ; CLINICAL-USE ; 1-BETA-D-ARABINOFURANOSYLCYTOSINE ; SYSTEMS ; ENANTIOSELECTIVITY ; CHEMOTHERAPY ; FLOXURIDINE
AbstractSubstrate recognition of Thermomyces lanuginosus lipase in the acylation of nucleosides was revealed through rational substrate engineering for the first time. T lanuginosus lipase displayed higher catalytic activities and excellent 5'-regioselectivities (94 -> 99%) in the acylation of ribonucleosides 1f-1j as compared to those in the acylation of 2'-deoxynucleosides la-le. The higher reaction rates and excellent 5'-regioselectivities might derive from a favorable hydrogen bonding between the 2'-hydroxyl group of 1f-1j and phenolic hydroxyl group of Tyr21 present in the hydrophilic region of the lipase. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000265465500015
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102329
Collection中国科学院大连化学物理研究所
Corresponding AuthorMinhua Zong; Ma D(马丁)
Affiliation1.S China Univ Technol, Lab Appl Biocatalysis, Guangzhou 510640, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Li, Ning,Zong, Min-Hua,Ma, Ding,et al. Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition[J]. JOURNAL OF BIOTECHNOLOGY,2009,140(3-4):250-253.
APA Li, Ning,Zong, Min-Hua,Ma, Ding,Minhua Zong,&马丁.(2009).Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition.JOURNAL OF BIOTECHNOLOGY,140(3-4),250-253.
MLA Li, Ning,et al."Thermomyces lanuginosus lipase-catalyzed regioselective acylation of nucleosides: Enzyme substrate recognition".JOURNAL OF BIOTECHNOLOGY 140.3-4(2009):250-253.
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