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题名: Macroporous Thermosensitive Imprinted Hydrogel for Recognition of Protein by Metal Coordinate Interaction
作者: Qin, Lei1;  He, Xi-Wen1;  Zhang, Wei1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
通讯作者: 张玉奎
刊名: ANALYTICAL CHEMISTRY
发表日期: 2009-09-01
DOI: 10.1021/ac900676t
卷: 81, 期:17, 页:7206-7216
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2;5
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A thermosensitive macroporous hydrogel showing selectivity for the lysozyme was developed by an imprinting procedure that is based on metal coordinate interaction. A metal chelate monomer [N-(4-vinyl)-benzyl iminodiacetic acid] forming coordination complex with the template protein in the presence of Cu ions co-polymerized with N-isopropylacrylamide and acrylamide, using N,N-methylenebisacrylamide as the cross-linker to prepare the thermosensitive protein-imprinted hydrogel. The synergetic combination of the smart property of the macroporous thermosensitive hydrogel with the merits of the coordinate interaction improved the selectivity and adsorption capacity, with respect to template lysozyme. The macropores were created by the frozen polymerization, and the influences of frozen polymerization and the chelate monomer content on the hydrogel affinity were investigated. The imprinted hydrogel can respond not only to external stimuli, but also to the template protein with a certain degree of shrinking. In recognition of the protein, the interaction of the imprinted thermosensitive hydrogel to the protein can be switched between the coordinate effect and the electrostatic effect by adding or not adding Cu ions. Finally, this imprinted hydrogel was used to purify the template lysozyme from the mixture of proteins and the real sample, which demonstrated its high selectivity.
关键词[WOS]: BOVINE SERUM-ALBUMIN ;  POLYMERS ;  ADSORPTION ;  GELS ;  CHROMATOGRAPHY ;  PURIFICATION ;  SEPARATION ;  TEMPLATES ;  MOLECULES ;  IONS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000269312000014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102381
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Qin, Lei,He, Xi-Wen,Zhang, Wei,et al. Macroporous Thermosensitive Imprinted Hydrogel for Recognition of Protein by Metal Coordinate Interaction[J]. ANALYTICAL CHEMISTRY,2009,81(17):7206-7216.
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