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Macroporous Thermosensitive Imprinted Hydrogel for Recognition of Protein by Metal Coordinate Interaction
Qin, Lei1; He, Xi-Wen1; Zhang, Wei1; Li, Wen-You1; Zhang, Yu-Kui1,2; Zhang YK(张玉奎)
Source PublicationANALYTICAL CHEMISTRY
2009-09-01
DOI10.1021/ac900676t
Volume81Issue:17Pages:7206-7216
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank2;5
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordBOVINE SERUM-ALBUMIN ; POLYMERS ; ADSORPTION ; GELS ; CHROMATOGRAPHY ; PURIFICATION ; SEPARATION ; TEMPLATES ; MOLECULES ; IONS
AbstractA thermosensitive macroporous hydrogel showing selectivity for the lysozyme was developed by an imprinting procedure that is based on metal coordinate interaction. A metal chelate monomer [N-(4-vinyl)-benzyl iminodiacetic acid] forming coordination complex with the template protein in the presence of Cu ions co-polymerized with N-isopropylacrylamide and acrylamide, using N,N-methylenebisacrylamide as the cross-linker to prepare the thermosensitive protein-imprinted hydrogel. The synergetic combination of the smart property of the macroporous thermosensitive hydrogel with the merits of the coordinate interaction improved the selectivity and adsorption capacity, with respect to template lysozyme. The macropores were created by the frozen polymerization, and the influences of frozen polymerization and the chelate monomer content on the hydrogel affinity were investigated. The imprinted hydrogel can respond not only to external stimuli, but also to the template protein with a certain degree of shrinking. In recognition of the protein, the interaction of the imprinted thermosensitive hydrogel to the protein can be switched between the coordinate effect and the electrostatic effect by adding or not adding Cu ions. Finally, this imprinted hydrogel was used to purify the template lysozyme from the mixture of proteins and the real sample, which demonstrated its high selectivity.
Language英语
URL查看原文
WOS IDWOS:000269312000014
Citation statistics
Cited Times:127[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102381
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang YK(张玉奎)
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Qin, Lei,He, Xi-Wen,Zhang, Wei,et al. Macroporous Thermosensitive Imprinted Hydrogel for Recognition of Protein by Metal Coordinate Interaction[J]. ANALYTICAL CHEMISTRY,2009,81(17):7206-7216.
APA Qin, Lei,He, Xi-Wen,Zhang, Wei,Li, Wen-You,Zhang, Yu-Kui,&张玉奎.(2009).Macroporous Thermosensitive Imprinted Hydrogel for Recognition of Protein by Metal Coordinate Interaction.ANALYTICAL CHEMISTRY,81(17),7206-7216.
MLA Qin, Lei,et al."Macroporous Thermosensitive Imprinted Hydrogel for Recognition of Protein by Metal Coordinate Interaction".ANALYTICAL CHEMISTRY 81.17(2009):7206-7216.
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