DICP OpenIR
Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography
Lin, Zian1,3; Yang, Fan1; He, Xiwen1; Zhao, Xiaomiao1; Zhang, Yukui1,2; Zian Lin
KeywordMolecular Imprinting Protein Hybrid Silica-based Monolith Organic Polymer-based Monolith
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2009-12-04
DOI10.1016/j.chroma.2009.10.025
Volume1216Issue:49Pages:8612-8622
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank2;5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCAPILLARY ELECTROCHROMATOGRAPHY ; POLYMER MATRIX ; SEPARATION ; BINDING ; ION ; MIMICS ; SITES ; BEADS
AbstractA novel type of macroporous molecularly imprinted hybrid silica monolithic column was first developed for recognition of proteins. The macroporous silica-based monolithic skeleton was synthesized in a 4.6 mm i.d. stainless steel column by a mild sol-gel process with methyltrimethoxysilane (MTMS) as a sole precursor, and then vinyl groups were introduced onto the surface of the silica skeleton by chemical modification of gamma-methacryloxypropyltrimethoxysilane (gamma-MAPS). Subsequently, the molecularly imprinted polymer (MIP) coating was copolymerized and anchored onto the surface of the silica monolith. Bovine serum albumin (BSA) and lysozyme (Lyz), which differ greatly in molecular size, isoelectric point, and charge, were representatively selected for imprinted templates to evaluate recognition property of the hybrid silica-based MIP monolith. Some important factors, such as template-monomer molar ratio, total monomer concentration and crosslinking density, were systematically investigated. Under the optimum conditions, the obtained hybrid silica-based MIP monolith showed higher binding affinity for template than its corresponding non-imprinted (NIP) monolith. The imprinted factor (IF) for BSA and Lyz reached 9.07 and 6.52, respectively. Moreover, the hybrid silica-based MIP monolith displayed favorable binding characteristics for template over competitive protein. Compared with the imprinted silica beads for stationary phase and in situ organic polymer-based hydrogel MIP monolith, the hybrid silica MIP monolith exhibited higher recognition, stability and lifetime. (C) 2009 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000272089200007
Citation statistics
Cited Times:80[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102383
Collection中国科学院大连化学物理研究所
Corresponding AuthorZian Lin
Affiliation1.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
3.Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Lin, Zian,Yang, Fan,He, Xiwen,et al. Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(49):8612-8622.
APA Lin, Zian,Yang, Fan,He, Xiwen,Zhao, Xiaomiao,Zhang, Yukui,&Zian Lin.(2009).Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1216(49),8612-8622.
MLA Lin, Zian,et al."Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1216.49(2009):8612-8622.
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