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题名: Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography
作者: Lin, Zian1, 3;  Yang, Fan1;  He, Xiwen1;  Zhao, Xiaomiao1;  Zhang, Yukui1, 2
通讯作者: Zian Lin
关键词: Molecular imprinting ;  Protein ;  Hybrid silica-based monolith ;  Organic polymer-based monolith
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-12-04
DOI: 10.1016/j.chroma.2009.10.025
卷: 1216, 期:49, 页:8612-8622
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2;5
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel type of macroporous molecularly imprinted hybrid silica monolithic column was first developed for recognition of proteins. The macroporous silica-based monolithic skeleton was synthesized in a 4.6 mm i.d. stainless steel column by a mild sol-gel process with methyltrimethoxysilane (MTMS) as a sole precursor, and then vinyl groups were introduced onto the surface of the silica skeleton by chemical modification of gamma-methacryloxypropyltrimethoxysilane (gamma-MAPS). Subsequently, the molecularly imprinted polymer (MIP) coating was copolymerized and anchored onto the surface of the silica monolith. Bovine serum albumin (BSA) and lysozyme (Lyz), which differ greatly in molecular size, isoelectric point, and charge, were representatively selected for imprinted templates to evaluate recognition property of the hybrid silica-based MIP monolith. Some important factors, such as template-monomer molar ratio, total monomer concentration and crosslinking density, were systematically investigated. Under the optimum conditions, the obtained hybrid silica-based MIP monolith showed higher binding affinity for template than its corresponding non-imprinted (NIP) monolith. The imprinted factor (IF) for BSA and Lyz reached 9.07 and 6.52, respectively. Moreover, the hybrid silica-based MIP monolith displayed favorable binding characteristics for template over competitive protein. Compared with the imprinted silica beads for stationary phase and in situ organic polymer-based hydrogel MIP monolith, the hybrid silica MIP monolith exhibited higher recognition, stability and lifetime. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY ELECTROCHROMATOGRAPHY ;  POLYMER MATRIX ;  SEPARATION ;  BINDING ;  ION ;  MIMICS ;  SITES ;  BEADS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000272089200007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102383
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
3.Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China

Recommended Citation:
Lin, Zian,Yang, Fan,He, Xiwen,et al. Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(49):8612-8622.
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