DICP OpenIR
Integrated Platform for Proteome Analysis with Combination of Protein and Peptide Separation via Online Digestion
Yuan, Huiming1,2; Zhang, Lihua1; Hou, Chunyan1,2; Zhu, Guijie1,2; Tao, Dingyin1,2; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
刊名ANALYTICAL CHEMISTRY
2009-11-01
DOI10.1021/ac900310y
81期:21页:8708-8714
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; TOP-DOWN PROTEOMICS ; ION-EXCHANGE ; IDENTIFICATION ; MICROREACTOR ; BOTTOM ; SYSTEM ; COLUMN ; MONOLITHS
英文摘要An integrated platform with the combination of protein and peptide separation was established via online protein digestion, by which proteins were first separated by a microcolumn packed with mixed weak anion and weak cation exchange (WAX/WCX) particles under a series of salt steps, online digested by a trypsin immobilized microenzymatic reactor (IMER), trapped and desalted by two parallel CS precolumns, separated by microreversed-phase liquid chromatography (mu RPLC) under a linear gradient of organic modifier concentration, and finally identified by electrospray ionization-MS/MS (ESI-MS/MS). To evaluate the performance of such a platform, a mixture of myoglobin, cytochrome c, bovine serum albumin (BSA), and alpha-casein, with mass ranging from 25 ng to 2 mu g, was analyzed. Compared to the methods by offline protein fractionation and shotgun based strategy, the analysis time, including sample preparation, digestion, desalting, separation, and detection, was shortened from ca. 30 to 5 h, and cytochrome c with abundance of 25 ng could be identified with improved sequence coverage. Furthermore, such an integrated platform was successfully applied into the analysis of proteins extracted from human lung cancer cells. Compared with the results obtained by the shotgun approach, the identified protein number was increased by 30%. All these results demonstrated that such an integrated approach would be an attractive alternative to commonly applied approaches for proteome research.
语种英语
原文出处查看原文
WOS记录号WOS:000276191900013
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102393
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Yuan, Huiming,Zhang, Lihua,Hou, Chunyan,et al. Integrated Platform for Proteome Analysis with Combination of Protein and Peptide Separation via Online Digestion[J]. ANALYTICAL CHEMISTRY,2009,81(21):8708-8714.
APA Yuan, Huiming.,Zhang, Lihua.,Hou, Chunyan.,Zhu, Guijie.,Tao, Dingyin.,...&张丽华.(2009).Integrated Platform for Proteome Analysis with Combination of Protein and Peptide Separation via Online Digestion.ANALYTICAL CHEMISTRY,81(21),8708-8714.
MLA Yuan, Huiming,et al."Integrated Platform for Proteome Analysis with Combination of Protein and Peptide Separation via Online Digestion".ANALYTICAL CHEMISTRY 81.21(2009):8708-8714.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yuan, Huiming]的文章
[Zhang, Lihua]的文章
[Hou, Chunyan]的文章
百度学术
百度学术中相似的文章
[Yuan, Huiming]的文章
[Zhang, Lihua]的文章
[Hou, Chunyan]的文章
必应学术
必应学术中相似的文章
[Yuan, Huiming]的文章
[Zhang, Lihua]的文章
[Hou, Chunyan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。