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题名: Evidence for the binding mode of porphyrins to G-quadruplex DNA
作者: Wei, Chunying1;  Jia, Guoqing2;  Zhou, Jun2;  Han, Gaoyi1;  Li, Can2
通讯作者: 李灿
刊名: PHYSICAL CHEMISTRY CHEMICAL PHYSICS
发表日期: 2009
DOI: 10.1039/b901027k
卷: 11, 期:20, 页:4025-4032
收录类别: SCI
文章类型: Article
部门归属: 5
项目归属: 503
产权排名: 2;2
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Physical ;  Physics, Atomic, Molecular & Chemical
研究领域[WOS]: Chemistry ;  Physics
英文摘要: Interactions of porphyrin derivatives 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin (TMPyP4) and 5,10,15,20-tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with human telomeric AG(3)(T(2)AG(3))(3) G-quadruplex DNAs in 150 mM K(+)-containing buffer in the presence or absence of 40% molecular crowding agent poly(ethylene glycol) (PEG 200) were studied by absorption titration fitting and time-resolved fluorescence spectroscopy. The results show that two TMPyP4 (or TPrPyP4) molecules bind to antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3) G-quadruplex by end-stacking and outside groove binding modes in the absence of PEG. Interestingly, in the presence of PEG one porphyrin molecule is stacked between two parallel AG(3)(T(2)AG(3))(3) G-quadruplexes to form a sandwich structure, another porphyrin molecule is bound to the groove of the G-quadruplex. The interactions of TMPyP4 with different structures of AG(3)(T(2)AG(3))(3) G-quadruplex are non cooperative, the binding constants of two independent binding sites are 1.07 x 10(6) and 4.42 x 10(8) M(-1) for an antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3), 8.67 x 10(5) and 2.26 x 10(8) M(-1) for parallel-stranded AG(3)(T(2)AG(3))(3) G-quadruplex. Conversely, the two binding sites are cooperative for TPrPyP4, the apparent association constants are 5.58 x 10(6) and 1.24 x 10(7) M(-1) for parallel-stranded and antiparallel/parallel hybrid structures of AG(3)(T(2)AG(3))(3) G-quadruplex, respectively.
关键词[WOS]: TIME-RESOLVED FLUORESCENCE ;  INTRAMOLECULAR G-QUADRUPLEX ;  G-QUARTET DNA ;  CATIONIC PORPHYRIN ;  STRUCTURAL BASIS ;  TELOMERIC DNA ;  CRYSTAL-STRUCTURES ;  LIGAND-BINDING ;  DRUG DESIGN ;  COMPLEXES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000266065400026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102487
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanxi Univ, Key Lab Chem Biol & Mol Engn, Minist Educ, Inst Mol Sci, Taiyuan 030006, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China

Recommended Citation:
Wei, Chunying,Jia, Guoqing,Zhou, Jun,et al. Evidence for the binding mode of porphyrins to G-quadruplex DNA[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS,2009,11(20):4025-4032.
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