DICP OpenIR
Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics
Zhou, Jun1,2; Wei, Chunying1,3; Jia, Guoqing1,2; Wang, Xiuli1,2; Feng, Zhaochi1; Li, Can1; Li C(李灿)
关键词G-quadruplex Duplex Structural Interconversion Molecular Crowding
刊名BIOCHIMIE
2009-09-01
DOI10.1016/j.biochi.2009.05.014
91期:9页:1104-1111
收录类别SCI
文章类型Article
部门归属5
项目归属503
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]RESONANCE ENERGY-TRANSFER ; G-QUARTET FORMATION ; MOLECULAR CROWDING CONDITIONS ; I-MOTIF STRUCTURES ; BINDING-PROTEIN ; COMPLEMENTARY STRAND ; DNA QUADRUPLEXES ; BETA-SUBUNIT ; EQUILIBRIUM ; COMPETITION
英文摘要The structural interconversion between the G-quadruplex and duplex in vivo is an important subject. In the present study, we used human telomeric DNA duplex composed of GGG(TTAGGG)(3)/CCC(TAACCC)(3) as a model system to investigate its properties under near physiological conditions by spectroscopic methods. Circular dichroism and fluorescence spectra demonstrated that G-quadruplex structure can be formed from duplex at near physiological pH (pH 7.4), salt concentration (150 mM K(+)), and temperature (37 degrees C) in the presence of molecular crowding agent PEG (400 g/l), whereas the G-quadruplex structure cannot be formed at 25 degrees C in buffer containing 150 mM K(+) in the presence of PEG. It is found that the formation rate of G-quadruplex structure depends on the temperature and the concentrations of both PEG and K(+). This work suggests that human telomeric G-quadruplex structure may be potentially formed from Watson-Crick duplex in vivo. (C) 2009 Elsevier Masson SAS. All rights reserved.
语种英语
原文出处查看原文
WOS记录号WOS:000269654100006
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102511
专题中国科学院大连化学物理研究所
通讯作者Li C(李灿)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Shanxi Univ, Inst Mol Sci, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Jun,Wei, Chunying,Jia, Guoqing,et al. Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics[J]. BIOCHIMIE,2009,91(9):1104-1111.
APA Zhou, Jun.,Wei, Chunying.,Jia, Guoqing.,Wang, Xiuli.,Feng, Zhaochi.,...&李灿.(2009).Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics.BIOCHIMIE,91(9),1104-1111.
MLA Zhou, Jun,et al."Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics".BIOCHIMIE 91.9(2009):1104-1111.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhou, Jun]的文章
[Wei, Chunying]的文章
[Jia, Guoqing]的文章
百度学术
百度学术中相似的文章
[Zhou, Jun]的文章
[Wei, Chunying]的文章
[Jia, Guoqing]的文章
必应学术
必应学术中相似的文章
[Zhou, Jun]的文章
[Wei, Chunying]的文章
[Jia, Guoqing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。