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题名: Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics
作者: Zhou, Jun1, 2;  Wei, Chunying1, 3;  Jia, Guoqing1, 2;  Wang, Xiuli1, 2;  Feng, Zhaochi1;  Li, Can1
通讯作者: 李灿
关键词: G-quadruplex ;  Duplex ;  Structural interconversion ;  Molecular crowding
刊名: BIOCHIMIE
发表日期: 2009-09-01
DOI: 10.1016/j.biochi.2009.05.014
卷: 91, 期:9, 页:1104-1111
收录类别: SCI
文章类型: Article
部门归属: 5
项目归属: 503
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: The structural interconversion between the G-quadruplex and duplex in vivo is an important subject. In the present study, we used human telomeric DNA duplex composed of GGG(TTAGGG)(3)/CCC(TAACCC)(3) as a model system to investigate its properties under near physiological conditions by spectroscopic methods. Circular dichroism and fluorescence spectra demonstrated that G-quadruplex structure can be formed from duplex at near physiological pH (pH 7.4), salt concentration (150 mM K(+)), and temperature (37 degrees C) in the presence of molecular crowding agent PEG (400 g/l), whereas the G-quadruplex structure cannot be formed at 25 degrees C in buffer containing 150 mM K(+) in the presence of PEG. It is found that the formation rate of G-quadruplex structure depends on the temperature and the concentrations of both PEG and K(+). This work suggests that human telomeric G-quadruplex structure may be potentially formed from Watson-Crick duplex in vivo. (C) 2009 Elsevier Masson SAS. All rights reserved.
关键词[WOS]: RESONANCE ENERGY-TRANSFER ;  G-QUARTET FORMATION ;  MOLECULAR CROWDING CONDITIONS ;  I-MOTIF STRUCTURES ;  BINDING-PROTEIN ;  COMPLEMENTARY STRAND ;  DNA QUADRUPLEXES ;  BETA-SUBUNIT ;  EQUILIBRIUM ;  COMPETITION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000269654100006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102511
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Shanxi Univ, Inst Mol Sci, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China

Recommended Citation:
Zhou, Jun,Wei, Chunying,Jia, Guoqing,et al. Human telomeric G-quadruplex formed from duplex under near physiological conditions: Spectroscopic evidence and kinetics[J]. BIOCHIMIE,2009,91(9):1104-1111.
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