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The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation
Tang, Wei1,2; Zhang, Sufang1; Wang, Qian1; Tan, Haidong1; Zhao, Zongbao Kent1; Zhao ZB(赵宗保)
关键词Lipomyces Starkeyi Isocitrate Dehydrogenase Oleaginous Yeast Real-time Pcr
刊名CANADIAN JOURNAL OF MICROBIOLOGY
2009-09-01
DOI10.1139/W09-063
55期:9页:1062-1069
收录类别SCI
文章类型Article
部门归属18
项目归属1816
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
关键词[WOS]RHODOSPORIDIUM-TORULOIDES CBS-14 ; BINDING-SITES ; CITRATE LYASE ; MICROORGANISMS ; GLUCOSE ; ATP ; TRIGLYCERIDES ; OPTIMIZATION ; EXPRESSION ; LIPOLYTICA
英文摘要The oleaginous yeast Lipomyces starkeyi can accumulate intracellular lipids to over 60% of its cell dry mass under a nitrogen-limited condition. We showed that extracellular and intracellular citrate concentrations of L. starkeyi AS 2.1560 increased and the nicotinamide adenine dinucleotide - isocitrate dehydrogenase (NAD(+)-IDH) activity decreased at the beginning of the lipid accumulation, suggesting that the attenuation of the NAD(+)-IDH activity might initiate lipid storage. We next cloned the IDH gene by the methods of degenerate PCR and rapid amplification of cDNA ends. Phylogenetic analyses of the evolutionary relationships among LsIDH1, LsIDH2, and other yeast NAD(+)-IDHs revealed that the L. starkeyi IDH had a closer relationship with the IDHs of Yarrowia lipolytica. Further real-time PCR analysis showed that the expression levels of both LsIDH1 and LsIDH2 decreased concurrently with the evolution of cellular lipids. Our data should be valuable for understanding the biology of oleaginous yeasts and for further strain engineering of L. starkeyi.
语种英语
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WOS记录号WOS:000271315900005
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102549
专题中国科学院大连化学物理研究所
通讯作者Zhao ZB(赵宗保)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Tang, Wei,Zhang, Sufang,Wang, Qian,et al. The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2009,55(9):1062-1069.
APA Tang, Wei,Zhang, Sufang,Wang, Qian,Tan, Haidong,Zhao, Zongbao Kent,&赵宗保.(2009).The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation.CANADIAN JOURNAL OF MICROBIOLOGY,55(9),1062-1069.
MLA Tang, Wei,et al."The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation".CANADIAN JOURNAL OF MICROBIOLOGY 55.9(2009):1062-1069.
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