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题名: The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation
作者: Tang, Wei1, 2;  Zhang, Sufang1;  Wang, Qian1;  Tan, Haidong1;  Zhao, Zongbao Kent1
通讯作者: 赵宗保
关键词: Lipomyces starkeyi ;  isocitrate dehydrogenase ;  oleaginous yeast ;  real-time PCR
刊名: CANADIAN JOURNAL OF MICROBIOLOGY
发表日期: 2009-09-01
DOI: 10.1139/W09-063
卷: 55, 期:9, 页:1062-1069
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Immunology ;  Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Immunology ;  Microbiology
英文摘要: The oleaginous yeast Lipomyces starkeyi can accumulate intracellular lipids to over 60% of its cell dry mass under a nitrogen-limited condition. We showed that extracellular and intracellular citrate concentrations of L. starkeyi AS 2.1560 increased and the nicotinamide adenine dinucleotide - isocitrate dehydrogenase (NAD(+)-IDH) activity decreased at the beginning of the lipid accumulation, suggesting that the attenuation of the NAD(+)-IDH activity might initiate lipid storage. We next cloned the IDH gene by the methods of degenerate PCR and rapid amplification of cDNA ends. Phylogenetic analyses of the evolutionary relationships among LsIDH1, LsIDH2, and other yeast NAD(+)-IDHs revealed that the L. starkeyi IDH had a closer relationship with the IDHs of Yarrowia lipolytica. Further real-time PCR analysis showed that the expression levels of both LsIDH1 and LsIDH2 decreased concurrently with the evolution of cellular lipids. Our data should be valuable for understanding the biology of oleaginous yeasts and for further strain engineering of L. starkeyi.
关键词[WOS]: RHODOSPORIDIUM-TORULOIDES CBS-14 ;  BINDING-SITES ;  CITRATE LYASE ;  MICROORGANISMS ;  GLUCOSE ;  ATP ;  TRIGLYCERIDES ;  OPTIMIZATION ;  EXPRESSION ;  LIPOLYTICA
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000271315900005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102549
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Tang, Wei,Zhang, Sufang,Wang, Qian,et al. The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2009,55(9):1062-1069.
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