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The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation
Tang, Wei1,2; Zhang, Sufang1; Wang, Qian1; Tan, Haidong1; Zhao, Zongbao Kent1; Zhao ZB(赵宗保)
KeywordLipomyces Starkeyi Isocitrate Dehydrogenase Oleaginous Yeast Real-time Pcr
Source PublicationCANADIAN JOURNAL OF MICROBIOLOGY
2009-09-01
DOI10.1139/W09-063
Volume55Issue:9Pages:1062-1069
Indexed BySCI
SubtypeArticle
Department18
Funding Project1816
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
WOS KeywordRHODOSPORIDIUM-TORULOIDES CBS-14 ; BINDING-SITES ; CITRATE LYASE ; MICROORGANISMS ; GLUCOSE ; ATP ; TRIGLYCERIDES ; OPTIMIZATION ; EXPRESSION ; LIPOLYTICA
AbstractThe oleaginous yeast Lipomyces starkeyi can accumulate intracellular lipids to over 60% of its cell dry mass under a nitrogen-limited condition. We showed that extracellular and intracellular citrate concentrations of L. starkeyi AS 2.1560 increased and the nicotinamide adenine dinucleotide - isocitrate dehydrogenase (NAD(+)-IDH) activity decreased at the beginning of the lipid accumulation, suggesting that the attenuation of the NAD(+)-IDH activity might initiate lipid storage. We next cloned the IDH gene by the methods of degenerate PCR and rapid amplification of cDNA ends. Phylogenetic analyses of the evolutionary relationships among LsIDH1, LsIDH2, and other yeast NAD(+)-IDHs revealed that the L. starkeyi IDH had a closer relationship with the IDHs of Yarrowia lipolytica. Further real-time PCR analysis showed that the expression levels of both LsIDH1 and LsIDH2 decreased concurrently with the evolution of cellular lipids. Our data should be valuable for understanding the biology of oleaginous yeasts and for further strain engineering of L. starkeyi.
Language英语
URL查看原文
WOS IDWOS:000271315900005
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102549
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao ZB(赵宗保)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Tang, Wei,Zhang, Sufang,Wang, Qian,et al. The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2009,55(9):1062-1069.
APA Tang, Wei,Zhang, Sufang,Wang, Qian,Tan, Haidong,Zhao, Zongbao Kent,&赵宗保.(2009).The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation.CANADIAN JOURNAL OF MICROBIOLOGY,55(9),1062-1069.
MLA Tang, Wei,et al."The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation".CANADIAN JOURNAL OF MICROBIOLOGY 55.9(2009):1062-1069.
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