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Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation
Liu, Hongwei2; Zhao, Xin1; Wang, Fangjun2; Li, Yonghong1; Jiang, Xining2; Ye, Mingliang2; Zhao, Zongbao K.1; Zou, Hanfa1,2; Zhao ZB(赵宗保); Zou HF(邹汉法)
关键词Oleaginous Microorganism Rhodosporidium Toruloides Nitrogen Deficiency 2d-lc-ms/ms Proteomics
刊名YEAST
2009-10-01
DOI10.1002/yea.1706
26期:10页:553-566
收录类别SCI
文章类型Article
部门归属18
项目归属1809,1816
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology
关键词[WOS]YEAST YARROWIA-LIPOLYTICA ; SACCHAROMYCES-CEREVISIAE ; MASS-SPECTROMETRY ; OLEAGINOUS YEAST ; LIPOMYCES-STARKEYI ; BODY FORMATION ; CARBON SOURCE ; PROTEIN ; NITROGEN ; GLUCOSE
英文摘要Intracellular lipid accumulation is a common biological process for some eukaryotic microorganisms under specific growth conditions, yet study on this phenomenon at an '-omics' level remains rare. In this study we induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides by transferring cells into a nitrogen-limited medium and performed a comparative and semi-quantitative proteomic analysis of cell samples obtained thereafter by a 2D-LC-MS/MS approach. A total of 184 proteins were identified, based on the database of yeast Saccharomyces cerevisiae. Semi-quantitative analysis suggested that 46 proteins were notably changed during the lipid production process. Among them, seven, three and four proteins were significantly upregulated only at the late stage, the early stage and both stages, respectively. There were 26 proteins drastically downregulated at both stages. The majority of the downregulated proteins are related to protein metabolism and carbohydrate metabolism, whereas the upregulated proteins are mainly involved in alternative nitrogen sources metabolism and lipid biosynthesis. Our data indicated that a nitrogen deficiency environment had a key impact on cellular metabolism that likely stimulated the lipid accumulation process by R. toruloides. This work provids valuable information for further exploration of the molecular mechanism of cellular lipid metabolism and should be of great interest in oleaginous microorganisms engineering. Copyright (C) 2009 John Wiley & Sons, Ltd.
语种英语
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WOS记录号WOS:000270859500003
引用统计
被引频次:39[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102553
专题中国科学院大连化学物理研究所
通讯作者Zhao ZB(赵宗保); Zou HF(邹汉法)
作者单位1.CAS, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.CAS, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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Liu, Hongwei,Zhao, Xin,Wang, Fangjun,et al. Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation[J]. YEAST,2009,26(10):553-566.
APA Liu, Hongwei.,Zhao, Xin.,Wang, Fangjun.,Li, Yonghong.,Jiang, Xining.,...&邹汉法.(2009).Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation.YEAST,26(10),553-566.
MLA Liu, Hongwei,et al."Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation".YEAST 26.10(2009):553-566.
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