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题名: Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation
作者: Liu, Hongwei2;  Zhao, Xin1;  Wang, Fangjun2;  Li, Yonghong1;  Jiang, Xining2;  Ye, Mingliang2;  Zhao, Zongbao K.1;  Zou, Hanfa1, 2
通讯作者: 赵宗保 ;  邹汉法
关键词: oleaginous microorganism ;  Rhodosporidium toruloides ;  nitrogen deficiency ;  2D-LC-MS/MS ;  proteomics
刊名: YEAST
发表日期: 2009-10-01
DOI: 10.1002/yea.1706
卷: 26, 期:10, 页:553-566
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809,1816
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
英文摘要: Intracellular lipid accumulation is a common biological process for some eukaryotic microorganisms under specific growth conditions, yet study on this phenomenon at an '-omics' level remains rare. In this study we induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides by transferring cells into a nitrogen-limited medium and performed a comparative and semi-quantitative proteomic analysis of cell samples obtained thereafter by a 2D-LC-MS/MS approach. A total of 184 proteins were identified, based on the database of yeast Saccharomyces cerevisiae. Semi-quantitative analysis suggested that 46 proteins were notably changed during the lipid production process. Among them, seven, three and four proteins were significantly upregulated only at the late stage, the early stage and both stages, respectively. There were 26 proteins drastically downregulated at both stages. The majority of the downregulated proteins are related to protein metabolism and carbohydrate metabolism, whereas the upregulated proteins are mainly involved in alternative nitrogen sources metabolism and lipid biosynthesis. Our data indicated that a nitrogen deficiency environment had a key impact on cellular metabolism that likely stimulated the lipid accumulation process by R. toruloides. This work provids valuable information for further exploration of the molecular mechanism of cellular lipid metabolism and should be of great interest in oleaginous microorganisms engineering. Copyright (C) 2009 John Wiley & Sons, Ltd.
关键词[WOS]: YEAST YARROWIA-LIPOLYTICA ;  SACCHAROMYCES-CEREVISIAE ;  MASS-SPECTROMETRY ;  OLEAGINOUS YEAST ;  LIPOMYCES-STARKEYI ;  BODY FORMATION ;  CARBON SOURCE ;  PROTEIN ;  NITROGEN ;  GLUCOSE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000270859500003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102553
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.CAS, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.CAS, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Liu, Hongwei,Zhao, Xin,Wang, Fangjun,et al. Comparative proteomic analysis of Rhodosporidium toruloides during lipid accumulation[J]. YEAST,2009,26(10):553-566.
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