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题名: Synthesis and Application of Prenyl-Derived Photoaffinity Probes
作者: Li Lingdong1, 2;  Tang Wei1, 2;  Zhao Zongbao1
通讯作者: 赵宗保
关键词: isoprenoid chain ;  chemical probe ;  photoaffinity labeling ;  click chemistry ;  chemical proteomics
刊名: CHINESE JOURNAL OF CHEMISTRY
发表日期: 2009-07-01
卷: 27, 期:7, 页:1391-1396
收录类别: SCI ;  IC
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Three photoaffinity probes containing isoprenoid chains and an azide group were synthesized using one-pot coupling reaction as the key step. The capability of these probes as labeling agents for isoprenoid chain-interacting proteins from Saccharomyces cerevisiae proteome was validated by photoaffinity reaction and "click" conjunction with the biotin reporter followed by streptavidin blot analysis.
关键词[WOS]: GERANYLGERANYLTRANSFERASE TYPE-I ;  HISTONE DEACETYLASE COMPLEXES ;  PROTEIN FARNESYLTRANSFERASE ;  FARNESYL TRANSFERASE ;  DIPHOSPHATE ANALOGS ;  ESTERS ;  PRENYLTRANSFERASES ;  CYCLOADDITION ;  BIOCHEMISTRY ;  INHIBITORS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000268625200031
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102571
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Liaoning, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Li Lingdong,Tang Wei,Zhao Zongbao. Synthesis and Application of Prenyl-Derived Photoaffinity Probes[J]. CHINESE JOURNAL OF CHEMISTRY,2009,27(7):1391-1396.
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