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题名: Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry
作者: Cai, Xiaoming1;  Zou, Lijuan1, 2;  Dong, Jun1;  Zhao, Lili2;  Wang, Yuanyuan2;  Xu, Qing1;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1
通讯作者: 梁鑫淼
关键词: Hydrophilic interaction liquid chromatography ;  Plasma ;  Metabonomics ;  Highly polar metabolites
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2009-09-14
DOI: 10.1016/j.aca.2009.01.054
卷: 650, 期:1, 页:10-15
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Highly polar metabolites are abundant in human plasma. Analysis of these compounds by standard reverse-phase chromatography is difficult. Highly polar metabolites were extracted by a two-step method. They were analyzed by ultra-performance hydrophilic interaction chromatography (HILIC) coupled to quadrupole-time of flight mass spectrometer detection technology. The middle and low polar fraction were also analyzed using a standard C18 column. Data were extracted by Markerlynx, and analyzed by multivariate statistical methods to discriminate human plasma samples by sex and age. The results not only showed good classification of samples by sex and age, but also revealed new potential biomarkers not revealed by standard RPLC/MS analysis. This study showed that HILIC is an effective technique to analyze highly polar metabolites for metabonomic studies. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: MULTIVARIATE STATISTICAL-ANALYSIS ;  PROTEIN PRECIPITATION ;  RAT URINE ;  METABONOMICS ;  SEPARATION ;  MS ;  SPECTROSCOPY ;  AGE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000270003800003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102583
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China
2.Dalian Med Univ, Hosp 2, Dalian 116023, Peoples R China

Recommended Citation:
Cai, Xiaoming,Zou, Lijuan,Dong, Jun,et al. Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2009,650(1):10-15.
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