DICP OpenIR
Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry
Cai, Xiaoming1; Zou, Lijuan1,2; Dong, Jun1; Zhao, Lili2; Wang, Yuanyuan2; Xu, Qing1; Xue, Xingya1; Zhang, Xiuli1; Liang, Xinmiao1; Liang XM(梁鑫淼)
KeywordHydrophilic Interaction Liquid Chromatography Plasma Metabonomics Highly Polar Metabolites
Source PublicationANALYTICA CHIMICA ACTA
2009-09-14
DOI10.1016/j.aca.2009.01.054
Volume650Issue:1Pages:10-15
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMULTIVARIATE STATISTICAL-ANALYSIS ; PROTEIN PRECIPITATION ; RAT URINE ; METABONOMICS ; SEPARATION ; MS ; SPECTROSCOPY ; AGE
AbstractHighly polar metabolites are abundant in human plasma. Analysis of these compounds by standard reverse-phase chromatography is difficult. Highly polar metabolites were extracted by a two-step method. They were analyzed by ultra-performance hydrophilic interaction chromatography (HILIC) coupled to quadrupole-time of flight mass spectrometer detection technology. The middle and low polar fraction were also analyzed using a standard C18 column. Data were extracted by Markerlynx, and analyzed by multivariate statistical methods to discriminate human plasma samples by sex and age. The results not only showed good classification of samples by sex and age, but also revealed new potential biomarkers not revealed by standard RPLC/MS analysis. This study showed that HILIC is an effective technique to analyze highly polar metabolites for metabonomic studies. (C) 2009 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000270003800003
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102583
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China
2.Dalian Med Univ, Hosp 2, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Cai, Xiaoming,Zou, Lijuan,Dong, Jun,et al. Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2009,650(1):10-15.
APA Cai, Xiaoming.,Zou, Lijuan.,Dong, Jun.,Zhao, Lili.,Wang, Yuanyuan.,...&梁鑫淼.(2009).Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry.ANALYTICA CHIMICA ACTA,650(1),10-15.
MLA Cai, Xiaoming,et al."Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry".ANALYTICA CHIMICA ACTA 650.1(2009):10-15.
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