中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Purification of alkaloids from Corydalis yanhusuo W. T. Wang using preparative 2-D HPLC
作者: Zhang, Jing1;  Jin, Yu2;  Liu, Yanfang1;  Mao, Yuansheng1;  Feng, Jiatao1;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1, 2
通讯作者: 梁鑫淼
关键词: Alkaloids ;  Corydalis yanhusuo W. T. Wang ;  pH values differences ;  Preparative parallel HPLC ;  Two-dimensional HPLC
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-05-01
DOI: 10.1002/jssc.200800729
卷: 32, 期:9, 页:1401-1406
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Two-dimensional preparative multi-channel parallel high performance liquid chromatography was successfully applied for the first time to isolate and purify alkaloids from Corydalis yanhusuo. The experiments were performed in off-line mode using the same preparative chromatographic column with pH 3.5 in the first and pH 10.0 in the second separation dimension. In the preparative process, UV-triggered fraction collection was used in the first dimension while UV and MS-triggered collection were used in the second dimension for reasons of sensitivity and complementarity. Two pure compounds and nine fractions were obtained in the first dimension. Then two representative fractions were further purified in the second dimension and six pure compounds were obtained. The results demonstrated that this procedure is an effective approach for the preparative isolation and purification of alkaloids from Corydalis yanhusuo. Based on the different pH values of the mobile phase in this method, it is also suitable for the preparative isolation and purification of other compounds from TCMs which are sensitive to the pH of the solutions. Moreover, this method will be a promising tool for the purification of low content compounds from natural products.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  COUNTER-CURRENT CHROMATOGRAPHY ;  COMBINATORIAL LIBRARIES ;  COLUMN CHROMATOGRAPHY ;  SEPARATION ;  FLAVONOIDS ;  SYSTEM ;  LEAVES ;  MS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000266341200017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102601
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

Recommended Citation:
Zhang, Jing,Jin, Yu,Liu, Yanfang,et al. Purification of alkaloids from Corydalis yanhusuo W. T. Wang using preparative 2-D HPLC[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(9):1401-1406.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhang, Jing]'s Articles
 [Jin, Yu]'s Articles
 [Liu, Yanfang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhang, Jing]‘s Articles
 [Jin, Yu]‘s Articles
 [Liu, Yanfang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace