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Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang
Zhang, Jing1; Jin, Yu; Liu, Yanfang1; Xiao, Yuansheng1; Feng, Jiatao1; Xue, Xingya1; Zhang, Xiuli1; Liang, Xinmiao1,2; Liang XM(梁鑫淼)
KeywordAlkaloids Corydalis Yanhusuo W. T. W.ng Different Ph Rplc-rplc Traditional Chinese Medicines
Source PublicationJOURNAL OF SEPARATION SCIENCE
2009-06-01
DOI10.1002/jssc.200800703
Volume32Issue:12Pages:2084-2089
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY MASS-SPECTROMETRY ; CHINESE MEDICINE ; QUANTITATIVE-DETERMINATION ; TETRAHYDROPALMATINE ; PROTOBERBERINE ; PEPTIDES ; ACONITUM ; HPLC ; MS
AbstractAn effective method utilizing the same RP chromatographic column with different pH in first and second LC dimensions has been developed for separation of the basic compounds from traditional Chinese medicines (TCMs). In this work, the alkaloids in Corydalis yanhusuo which is an important TCM were selected as a model to develop the method. The additives and pH values of the mobile phase were optimized in this work. To investigate the feasibility of this method, off-line mode separation was performed in the experiments. According to the UV-absorption intensity, there were eight fractions collected in acidic conditions. All the fractions were analyzed in basic conditions. The results showed that the chromatographic selectivities were significantly different in the separations performed with acidic and alkaline elution systems. Complementary separation was achieved in this work. It is demonstrated that this method would be an effective tool for alkaloids research. Based on the different pH of the mobile phase in this method, it could also be suitable to analyze compounds which were sensible to the pH of the solution.
Language英语
URL查看原文
WOS IDWOS:000267811600011
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102603
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jing,Jin, Yu,Liu, Yanfang,et al. Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(12):2084-2089.
APA Zhang, Jing.,Jin, Yu.,Liu, Yanfang.,Xiao, Yuansheng.,Feng, Jiatao.,...&梁鑫淼.(2009).Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang.JOURNAL OF SEPARATION SCIENCE,32(12),2084-2089.
MLA Zhang, Jing,et al."Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang".JOURNAL OF SEPARATION SCIENCE 32.12(2009):2084-2089.
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