DICP OpenIR
Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang
Zhang, Jing1; Jin, Yu; Liu, Yanfang1; Xiao, Yuansheng1; Feng, Jiatao1; Xue, Xingya1; Zhang, Xiuli1; Liang, Xinmiao1,2; Liang XM(梁鑫淼)
关键词Alkaloids Corydalis Yanhusuo W. T. W.ng Different Ph Rplc-rplc Traditional Chinese Medicines
刊名JOURNAL OF SEPARATION SCIENCE
2009-06-01
DOI10.1002/jssc.200800703
32期:12页:2084-2089
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY MASS-SPECTROMETRY ; CHINESE MEDICINE ; QUANTITATIVE-DETERMINATION ; TETRAHYDROPALMATINE ; PROTOBERBERINE ; PEPTIDES ; ACONITUM ; HPLC ; MS
英文摘要An effective method utilizing the same RP chromatographic column with different pH in first and second LC dimensions has been developed for separation of the basic compounds from traditional Chinese medicines (TCMs). In this work, the alkaloids in Corydalis yanhusuo which is an important TCM were selected as a model to develop the method. The additives and pH values of the mobile phase were optimized in this work. To investigate the feasibility of this method, off-line mode separation was performed in the experiments. According to the UV-absorption intensity, there were eight fractions collected in acidic conditions. All the fractions were analyzed in basic conditions. The results showed that the chromatographic selectivities were significantly different in the separations performed with acidic and alkaline elution systems. Complementary separation was achieved in this work. It is demonstrated that this method would be an effective tool for alkaloids research. Based on the different pH of the mobile phase in this method, it could also be suitable to analyze compounds which were sensible to the pH of the solution.
语种英语
原文出处查看原文
WOS记录号WOS:000267811600011
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102603
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Jing,Jin, Yu,Liu, Yanfang,et al. Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(12):2084-2089.
APA Zhang, Jing.,Jin, Yu.,Liu, Yanfang.,Xiao, Yuansheng.,Feng, Jiatao.,...&梁鑫淼.(2009).Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang.JOURNAL OF SEPARATION SCIENCE,32(12),2084-2089.
MLA Zhang, Jing,et al."Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang".JOURNAL OF SEPARATION SCIENCE 32.12(2009):2084-2089.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Jing]的文章
[Jin, Yu]的文章
[Liu, Yanfang]的文章
百度学术
百度学术中相似的文章
[Zhang, Jing]的文章
[Jin, Yu]的文章
[Liu, Yanfang]的文章
必应学术
必应学术中相似的文章
[Zhang, Jing]的文章
[Jin, Yu]的文章
[Liu, Yanfang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。