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题名: Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang
作者: Zhang, Jing1;  Jin, Yu;  Liu, Yanfang1;  Xiao, Yuansheng1;  Feng, Jiatao1;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1, 2
通讯作者: 梁鑫淼
关键词: Alkaloids ;  Corydalis yanhusuo W. T. Wang ;  Different pH ;  RPLC-RPLC ;  Traditional Chinese medicines
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-06-01
DOI: 10.1002/jssc.200800703
卷: 32, 期:12, 页:2084-2089
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An effective method utilizing the same RP chromatographic column with different pH in first and second LC dimensions has been developed for separation of the basic compounds from traditional Chinese medicines (TCMs). In this work, the alkaloids in Corydalis yanhusuo which is an important TCM were selected as a model to develop the method. The additives and pH values of the mobile phase were optimized in this work. To investigate the feasibility of this method, off-line mode separation was performed in the experiments. According to the UV-absorption intensity, there were eight fractions collected in acidic conditions. All the fractions were analyzed in basic conditions. The results showed that the chromatographic selectivities were significantly different in the separations performed with acidic and alkaline elution systems. Complementary separation was achieved in this work. It is demonstrated that this method would be an effective tool for alkaloids research. Based on the different pH of the mobile phase in this method, it could also be suitable to analyze compounds which were sensible to the pH of the solution.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ELECTROSPRAY MASS-SPECTROMETRY ;  CHINESE MEDICINE ;  QUANTITATIVE-DETERMINATION ;  TETRAHYDROPALMATINE ;  PROTOBERBERINE ;  PEPTIDES ;  ACONITUM ;  HPLC ;  MS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000267811600011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102603
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

Recommended Citation:
Zhang, Jing,Jin, Yu,Liu, Yanfang,et al. Two-dimensional RPLC-RPLC system with different pH in two dimensions for separation of alkaloids from Corydalis yanhusuo W. T. Wang[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(12):2084-2089.
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