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Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode
Zhang, Hui; Guo, Zhimou; Li, Wei; Feng, Jiatao; Xiao, Yuansheng; Zhang, Feifang; Xue, Xingya; Liang, Xinmiao; Liang XM(梁鑫淼)
关键词Flavonoids Hydrophilic Interaction Lc Licorice Preparative Hplc Triterpene Saponins
刊名JOURNAL OF SEPARATION SCIENCE
2009-02-01
DOI10.1002/jssc.200800526
32期:4页:526-535
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]HYDROPHILIC-INTERACTION CHROMATOGRAPHY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY MASS-SPECTROMETRY ; COUNTER-CURRENT CHROMATOGRAPHY ; HAIRY ROOT CULTURES ; ESI-MS SENSITIVITY ; GLYCYRRHIZA-URALENSIS ; REVERSED-PHASE ; POLAR COMPOUNDS ; DRUG DISCOVERY
英文摘要A four-channel preparative HPLC was employed to isolate and purify compounds from licorice extract. Two separation modes, RP and hydrophilic interaction LC (HILIC), were used in preparative HPLC. HILIC mode was adopted to resolve the purification of the compounds with similar hydrophobicity, which were co-eluted under RP mode. Using the two separation modes during the purification process, fifteen compounds were isolated from licorice extract. The results indicated that preparative HPLC performed under HILIC mode is an efficient method for the isolation and purification of compounds from natural products.
语种英语
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WOS记录号WOS:000264082100007
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102611
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Zhang, Hui,Guo, Zhimou,Li, Wei,et al. Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(4):526-535.
APA Zhang, Hui.,Guo, Zhimou.,Li, Wei.,Feng, Jiatao.,Xiao, Yuansheng.,...&梁鑫淼.(2009).Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode.JOURNAL OF SEPARATION SCIENCE,32(4),526-535.
MLA Zhang, Hui,et al."Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode".JOURNAL OF SEPARATION SCIENCE 32.4(2009):526-535.
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