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题名: Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode
作者: Zhang, Hui1;  Guo, Zhimou1;  Li, Wei1;  Feng, Jiatao1;  Xiao, Yuansheng1;  Zhang, Feifang1;  Xue, Xingya1;  Liang, Xinmiao1
通讯作者: 梁鑫淼
关键词: Flavonoids ;  Hydrophilic interaction LC ;  Licorice ;  Preparative HPLC ;  Triterpene saponins
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-02-01
DOI: 10.1002/jssc.200800526
卷: 32, 期:4, 页:526-535
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A four-channel preparative HPLC was employed to isolate and purify compounds from licorice extract. Two separation modes, RP and hydrophilic interaction LC (HILIC), were used in preparative HPLC. HILIC mode was adopted to resolve the purification of the compounds with similar hydrophobicity, which were co-eluted under RP mode. Using the two separation modes during the purification process, fifteen compounds were isolated from licorice extract. The results indicated that preparative HPLC performed under HILIC mode is an efficient method for the isolation and purification of compounds from natural products.
关键词[WOS]: HYDROPHILIC-INTERACTION CHROMATOGRAPHY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ELECTROSPRAY MASS-SPECTROMETRY ;  COUNTER-CURRENT CHROMATOGRAPHY ;  HAIRY ROOT CULTURES ;  ESI-MS SENSITIVITY ;  GLYCYRRHIZA-URALENSIS ;  REVERSED-PHASE ;  POLAR COMPOUNDS ;  DRUG DISCOVERY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000264082100007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102611
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Hui,Guo, Zhimou,Li, Wei,et al. Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(4):526-535.
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