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Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode
Zhang, Hui; Guo, Zhimou; Li, Wei; Feng, Jiatao; Xiao, Yuansheng; Zhang, Feifang; Xue, Xingya; Liang, Xinmiao; Liang XM(梁鑫淼)
KeywordFlavonoids Hydrophilic Interaction Lc Licorice Preparative Hplc Triterpene Saponins
Source PublicationJOURNAL OF SEPARATION SCIENCE
2009-02-01
DOI10.1002/jssc.200800526
Volume32Issue:4Pages:526-535
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordHYDROPHILIC-INTERACTION CHROMATOGRAPHY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY MASS-SPECTROMETRY ; COUNTER-CURRENT CHROMATOGRAPHY ; HAIRY ROOT CULTURES ; ESI-MS SENSITIVITY ; GLYCYRRHIZA-URALENSIS ; REVERSED-PHASE ; POLAR COMPOUNDS ; DRUG DISCOVERY
AbstractA four-channel preparative HPLC was employed to isolate and purify compounds from licorice extract. Two separation modes, RP and hydrophilic interaction LC (HILIC), were used in preparative HPLC. HILIC mode was adopted to resolve the purification of the compounds with similar hydrophobicity, which were co-eluted under RP mode. Using the two separation modes during the purification process, fifteen compounds were isolated from licorice extract. The results indicated that preparative HPLC performed under HILIC mode is an efficient method for the isolation and purification of compounds from natural products.
Language英语
URL查看原文
WOS IDWOS:000264082100007
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102611
Collection中国科学院大连化学物理研究所
Corresponding AuthorLiang XM(梁鑫淼)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Hui,Guo, Zhimou,Li, Wei,et al. Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(4):526-535.
APA Zhang, Hui.,Guo, Zhimou.,Li, Wei.,Feng, Jiatao.,Xiao, Yuansheng.,...&梁鑫淼.(2009).Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode.JOURNAL OF SEPARATION SCIENCE,32(4),526-535.
MLA Zhang, Hui,et al."Purification of flavonoids and triterpene saponins from the licorice extract using preparative HPLC under RP and HILIC mode".JOURNAL OF SEPARATION SCIENCE 32.4(2009):526-535.
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