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Hydrophilic Interaction Chromatography Based Enrichment of Glycopeptides by Using Click Maltose: A Matrix with High Selectivity and Glycosylation Heterogeneity Coverage
Yu, Long; Li, Xiuling; Guo, Zhimou; Zhang, Xiuli; Liang, Xinmiao; Liang XM(梁鑫淼)
关键词Analytical Methods Glycopeptides Glycoproteins Mass Spectrometry
刊名CHEMISTRY-A EUROPEAN JOURNAL
2009
DOI10.1002/chem.200902370
15期:46页:12618-12626
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]LECTIN AFFINITY-CHROMATOGRAPHY ; INTERACTION LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; PROTEIN GLYCOSYLATION ; IMMUNOGLOBULIN-G ; UNDERIVATIZED OLIGOSACCHARIDES ; GLYCOPROTEIN GLYCOSYLATION ; ALPHA-1-ACID GLYCOPROTEIN ; ZWITTERIONIC TYPE ; N-GLYCOPEPTIDES
英文摘要Glycosylation analysis based on mass spectrometry (MS) of glycopeptides requires the isolation of glycopeptides from complex glycoprotein digests to facilitate structural determination of the glycopeptides. To this end, hydrophilic interaction chromatography (HILIC)-based methods have been developed to selectively enrich glycopeptides by utilizing the hydrophilicity of the glycans. However, the application of these methods is limited by the medium selectivity of HILIC matrices. To improve the effectiveness of HILIC-based methods, we introduced a customized hydrophilic matrix named "click maltose" and characterized its selectivity and glycosylation heterogeneity coverage. In the selectivity assessment, the non-glycopeptides causing ion suppression to the glycopeptides were effectively removed by click maltose, leading to the identification of 27 glycopeptides in the fractions enriched from human serum immunoglobulin G digest, compared to 13 glycopeptides enriched using Sepharose CL-6B, a commercially available matrix. For the assessment of glycosylation heterogeneity coverage, more than 140 glycopeptides covering all the five glycosites of human serum alpha(1)-acid glycoprotein were captured using click maltose. Click maltose was synthesized by linking alkynyl-derivatized maltose to azide-derivatized silica through click chemistry. The resulting flexible saccharide chain structure remarkably enhances the hydrogen-bonding interactions between the glycans of the glycopeptides and the matrix, which are responsible for the increased selectivity and glycosylation heterogeneity coverage of click maltose.
语种英语
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WOS记录号WOS:000272509500013
引用统计
被引频次:117[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102677
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Yu, Long,Li, Xiuling,Guo, Zhimou,et al. Hydrophilic Interaction Chromatography Based Enrichment of Glycopeptides by Using Click Maltose: A Matrix with High Selectivity and Glycosylation Heterogeneity Coverage[J]. CHEMISTRY-A EUROPEAN JOURNAL,2009,15(46):12618-12626.
APA Yu, Long,Li, Xiuling,Guo, Zhimou,Zhang, Xiuli,Liang, Xinmiao,&梁鑫淼.(2009).Hydrophilic Interaction Chromatography Based Enrichment of Glycopeptides by Using Click Maltose: A Matrix with High Selectivity and Glycosylation Heterogeneity Coverage.CHEMISTRY-A EUROPEAN JOURNAL,15(46),12618-12626.
MLA Yu, Long,et al."Hydrophilic Interaction Chromatography Based Enrichment of Glycopeptides by Using Click Maltose: A Matrix with High Selectivity and Glycosylation Heterogeneity Coverage".CHEMISTRY-A EUROPEAN JOURNAL 15.46(2009):12618-12626.
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