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Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation
Liang, Yu2; Cong, Yongzheng2; Liang, Zhen; Zhang, Lihua1; Zhang, Yukui; Zhang LH(张丽华)
关键词Microchip Isoelectric Focusing Monolithic Immobilized Ph Gradient Materials Photopolymerization Proteins
刊名ELECTROPHORESIS
2009-12-01
DOI10.1002/elps.200900209
30期:23页:4034-4039
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]POLY(DIMETHYLSILOXANE) MICROFLUIDIC CHIP ; TEMPERATURE-GRADIENT ; GEL-ELECTROPHORESIS ; POLYACRYLAMIDE-GELS ; IMAGING DETECTION ; INTEGRATION ; COLUMN ; MICROCHANNELS ; ADAPTATION ; CHANNELS
英文摘要Monolithic immobilized pH gradient (M-IPG) materials were prepared in microchannles by photoinitiated polymerization of acrylamide, glycidylmethacrylate and Bis, followed by the attachment of focused Ampholine onto the surface of porous monoliths via epoxide groups. With M-IPG materials as matrix, FITC-labeled ribonuclease B, myoglobin and alpha-casein were well separated by microchip isoelectric focusing (mu CIEF) without carrier amphocytes (CAs) added in the buffer. Both chemical and pressure mobilization were applied to drive focused zones for LIF detection. Our experimental results showed that pressure mobilization was preferable with neglectable band broadening, and good peak shape and high detection sensitivity were obtained. All these results demonstrate that mu CIEF with M-IPG materials is not only an efficient mode for protein enrichment and separation but also attractive to couple with other CE modes to achieve multi-dimensional separation or MS for further identification, without the interference of mobile CAs.
语种英语
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WOS记录号WOS:000272928700006
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102701
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
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Liang, Yu,Cong, Yongzheng,Liang, Zhen,et al. Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation[J]. ELECTROPHORESIS,2009,30(23):4034-4039.
APA Liang, Yu,Cong, Yongzheng,Liang, Zhen,Zhang, Lihua,Zhang, Yukui,&张丽华.(2009).Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation.ELECTROPHORESIS,30(23),4034-4039.
MLA Liang, Yu,et al."Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation".ELECTROPHORESIS 30.23(2009):4034-4039.
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