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Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation
Liang, Yu2; Cong, Yongzheng2; Liang, Zhen; Zhang, Lihua1; Zhang, Yukui; Zhang LH(张丽华)
KeywordMicrochip Isoelectric Focusing Monolithic Immobilized Ph Gradient Materials Photopolymerization Proteins
Source PublicationELECTROPHORESIS
2009-12-01
DOI10.1002/elps.200900209
Volume30Issue:23Pages:4034-4039
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPOLY(DIMETHYLSILOXANE) MICROFLUIDIC CHIP ; TEMPERATURE-GRADIENT ; GEL-ELECTROPHORESIS ; POLYACRYLAMIDE-GELS ; IMAGING DETECTION ; INTEGRATION ; COLUMN ; MICROCHANNELS ; ADAPTATION ; CHANNELS
AbstractMonolithic immobilized pH gradient (M-IPG) materials were prepared in microchannles by photoinitiated polymerization of acrylamide, glycidylmethacrylate and Bis, followed by the attachment of focused Ampholine onto the surface of porous monoliths via epoxide groups. With M-IPG materials as matrix, FITC-labeled ribonuclease B, myoglobin and alpha-casein were well separated by microchip isoelectric focusing (mu CIEF) without carrier amphocytes (CAs) added in the buffer. Both chemical and pressure mobilization were applied to drive focused zones for LIF detection. Our experimental results showed that pressure mobilization was preferable with neglectable band broadening, and good peak shape and high detection sensitivity were obtained. All these results demonstrate that mu CIEF with M-IPG materials is not only an efficient mode for protein enrichment and separation but also attractive to couple with other CE modes to achieve multi-dimensional separation or MS for further identification, without the interference of mobile CAs.
Language英语
URL查看原文
WOS IDWOS:000272928700006
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102701
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liang, Yu,Cong, Yongzheng,Liang, Zhen,et al. Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation[J]. ELECTROPHORESIS,2009,30(23):4034-4039.
APA Liang, Yu,Cong, Yongzheng,Liang, Zhen,Zhang, Lihua,Zhang, Yukui,&张丽华.(2009).Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation.ELECTROPHORESIS,30(23),4034-4039.
MLA Liang, Yu,et al."Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation".ELECTROPHORESIS 30.23(2009):4034-4039.
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