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题名: Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation
作者: Liang, Yu2;  Cong, Yongzheng2;  Liang, Zhen;  Zhang, Lihua1;  Zhang, Yukui
通讯作者: 张丽华
关键词: Microchip isoelectric focusing ;  Monolithic immobilized pH gradient materials ;  Photopolymerization ;  Proteins
刊名: ELECTROPHORESIS
发表日期: 2009-12-01
DOI: 10.1002/elps.200900209
卷: 30, 期:23, 页:4034-4039
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Monolithic immobilized pH gradient (M-IPG) materials were prepared in microchannles by photoinitiated polymerization of acrylamide, glycidylmethacrylate and Bis, followed by the attachment of focused Ampholine onto the surface of porous monoliths via epoxide groups. With M-IPG materials as matrix, FITC-labeled ribonuclease B, myoglobin and alpha-casein were well separated by microchip isoelectric focusing (mu CIEF) without carrier amphocytes (CAs) added in the buffer. Both chemical and pressure mobilization were applied to drive focused zones for LIF detection. Our experimental results showed that pressure mobilization was preferable with neglectable band broadening, and good peak shape and high detection sensitivity were obtained. All these results demonstrate that mu CIEF with M-IPG materials is not only an efficient mode for protein enrichment and separation but also attractive to couple with other CE modes to achieve multi-dimensional separation or MS for further identification, without the interference of mobile CAs.
关键词[WOS]: POLY(DIMETHYLSILOXANE) MICROFLUIDIC CHIP ;  TEMPERATURE-GRADIENT ;  GEL-ELECTROPHORESIS ;  POLYACRYLAMIDE-GELS ;  IMAGING DETECTION ;  INTEGRATION ;  COLUMN ;  MICROCHANNELS ;  ADAPTATION ;  CHANNELS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000272928700006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102701
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Liang, Yu,Cong, Yongzheng,Liang, Zhen,et al. Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation[J]. ELECTROPHORESIS,2009,30(23):4034-4039.
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