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Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry
Ma, Chenfei1; Wang, Huahong2; Lu, Xin1; Wang, Hong2; Xu, Guowang1; Liu, Benye2; Xu GW(许国旺)
关键词Amorpha-4 11-diene Synthase Artemisia Annua L. Transgenic Terpenoid Metabolic Profiling
刊名METABOLOMICS
2009-12-01
DOI10.1007/s11306-009-0170-6
5期:4页:497-506
收录类别SCI
文章类型Article
部门归属18
项目归属1808
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Endocrinology & Metabolism
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; IN-VIVO TRANSFORMATIONS ; ANTIMALARIAL-DRUG ; DIHYDROARTEMISINIC ACID ; PLANTS ; BIOSYNTHESIS ; IDENTIFICATION ; SYNTHASE ; MALARIA ; GENE
英文摘要Amorpha-4,11-diene synthase (ADS) is a very important enzyme which catalyzes the committed step of artemisinin biosynthesis. In this work, two lines of transgenic Artemisia annua L. which ADS was over-expressed (line A9) and suppressed (line Amsi), respectively, were utilized. And the transgenic line GUS with beta-Glucuronidase gene was regarded as the control. Their terpenoid metabolic profiling was investigated by using GC x GC-TOFMS. The metabolic profiling method established included simple extraction, two-dimension separation and multivariate analysis. Partial least squares discriminant analysis (PLS-DA) was used to classify two transgenic lines and the control line. Eleven important compounds in classification were identified. Most of them were sesquiterpenoids including monoterpenoid, diterpenoid and four bioprecursors of artemsisnin. Compared with the control, artemisinin and bioprecursors in the line A9 increased as a result of over-expressing ADS. Borneol and phytol also increased in the line A9, but (E)-beta-farnesene and germacrene D were reversely altered. The result indicated that over-expression of the ADS affected not only artemisinin biosynthesis, but also the whole metabolic network of terpenoid. Compared with the line A9, no opposite change of artemisinin and related derivatives was observed in the line Amsi, the ADS inhibition had no significant effect on artemisinin biosynthesis in the line Amsi.
语种英语
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WOS记录号WOS:000272849800009
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102717
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China
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Ma, Chenfei,Wang, Huahong,Lu, Xin,et al. Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry[J]. METABOLOMICS,2009,5(4):497-506.
APA Ma, Chenfei.,Wang, Huahong.,Lu, Xin.,Wang, Hong.,Xu, Guowang.,...&许国旺.(2009).Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry.METABOLOMICS,5(4),497-506.
MLA Ma, Chenfei,et al."Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry".METABOLOMICS 5.4(2009):497-506.
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