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题名: Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry
作者: Ma, Chenfei1;  Wang, Huahong2;  Lu, Xin1;  Wang, Hong2;  Xu, Guowang1;  Liu, Benye2
通讯作者: 许国旺
关键词: Amorpha-4 ;  11-Diene synthase ;  Artemisia annua L. ;  Transgenic ;  Terpenoid ;  Metabolic profiling
刊名: METABOLOMICS
发表日期: 2009-12-01
DOI: 10.1007/s11306-009-0170-6
卷: 5, 期:4, 页:497-506
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
英文摘要: Amorpha-4,11-diene synthase (ADS) is a very important enzyme which catalyzes the committed step of artemisinin biosynthesis. In this work, two lines of transgenic Artemisia annua L. which ADS was over-expressed (line A9) and suppressed (line Amsi), respectively, were utilized. And the transgenic line GUS with beta-Glucuronidase gene was regarded as the control. Their terpenoid metabolic profiling was investigated by using GC x GC-TOFMS. The metabolic profiling method established included simple extraction, two-dimension separation and multivariate analysis. Partial least squares discriminant analysis (PLS-DA) was used to classify two transgenic lines and the control line. Eleven important compounds in classification were identified. Most of them were sesquiterpenoids including monoterpenoid, diterpenoid and four bioprecursors of artemsisnin. Compared with the control, artemisinin and bioprecursors in the line A9 increased as a result of over-expressing ADS. Borneol and phytol also increased in the line A9, but (E)-beta-farnesene and germacrene D were reversely altered. The result indicated that over-expression of the ADS affected not only artemisinin biosynthesis, but also the whole metabolic network of terpenoid. Compared with the line A9, no opposite change of artemisinin and related derivatives was observed in the line Amsi, the ADS inhibition had no significant effect on artemisinin biosynthesis in the line Amsi.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  IN-VIVO TRANSFORMATIONS ;  ANTIMALARIAL-DRUG ;  DIHYDROARTEMISINIC ACID ;  PLANTS ;  BIOSYNTHESIS ;  IDENTIFICATION ;  SYNTHASE ;  MALARIA ;  GENE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000272849800009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102717
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China

Recommended Citation:
Ma, Chenfei,Wang, Huahong,Lu, Xin,et al. Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry[J]. METABOLOMICS,2009,5(4):497-506.
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