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Rapid discrimination of single-nucleotide mismatches based on reciprocating flow on a compact disc microfluidic device
Li, Chunyu1,2; Li, Hongjing3; Qin, Jianhua1; Lin, Bingcheng1; Qin JH(秦建华); Lin BC(林炳承)
关键词Capillary Action Centrifugal Force Microfluidics Reciprocating Flow Single-nucleotide Mismatches
刊名ELECTROPHORESIS
2009-12-01
DOI10.1002/elps.200900305
30期:24页:4270-4276
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]OLIGONUCLEOTIDE MICROARRAYS ; DNA HYBRIDIZATION ; MUTATIONS ; ALLELE ; CD ; MICROCHIPS ; PLATFORM ; ARRAYS ; GENE ; CHIP
英文摘要In this study, a compact disc (CD) microfluidic device based on reciprocating flow is developed for the rapid discrimination of single-nucleotide mismatch (SNM). This device consists of a PDMS CD slab containing eight SNM assay units and a glass substrate immobilized with DNA probe array. The rapid SNM assay is realized by two-step controllable reciprocating flow in the microchannel, resulting from centrifugal force and capillary action as well. A perfect-match probe and three mismatched probes containing a SNM at various positions were designed to investigate the ability of this device to discriminate SNM. Based on the difference between the dissociation kinetics of perfect-match and mismatched duplexes, this CD device was able to discriminate SNM within 400 s. Moreover, only sub-microliter volume DNA samples (350 nL) and washing buffers (350 nL) were required per assay unit. The results indicate that this device has the potential to be used for fast, accurate gene diagnostics, especially for the detection of SNPs.
语种英语
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WOS记录号WOS:000273187800017
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102787
专题中国科学院大连化学物理研究所
通讯作者Qin JH(秦建华); Lin BC(林炳承)
作者单位1.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China
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GB/T 7714
Li, Chunyu,Li, Hongjing,Qin, Jianhua,et al. Rapid discrimination of single-nucleotide mismatches based on reciprocating flow on a compact disc microfluidic device[J]. ELECTROPHORESIS,2009,30(24):4270-4276.
APA Li, Chunyu,Li, Hongjing,Qin, Jianhua,Lin, Bingcheng,秦建华,&林炳承.(2009).Rapid discrimination of single-nucleotide mismatches based on reciprocating flow on a compact disc microfluidic device.ELECTROPHORESIS,30(24),4270-4276.
MLA Li, Chunyu,et al."Rapid discrimination of single-nucleotide mismatches based on reciprocating flow on a compact disc microfluidic device".ELECTROPHORESIS 30.24(2009):4270-4276.
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