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A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans
Ma, Hui; Jiang, Lei; Shi, Weiwei; Qin, Jianhua; Lin, Bingcheng; Qin JH(秦建华); Lin BC(林炳承)
关键词Biomems Diseases Microchannel Flow Neurophysiology
刊名BIOMICROFLUIDICS
2009-10-01
DOI10.1063/1.3274313
3期:4页:044114-1-044114-8
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Biophysics ; Nanoscience & Nanotechnology ; Physics, Fluids & Plasmas
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Science & Technology - Other Topics ; Physics
关键词[WOS]CAENORHABDITIS-ELEGANS ; ON-CHIP ; SYSTEM ; IMMOBILIZATION ; GENETICS ; NEURONS
英文摘要The soil dwelling nematode Caenorhabditis elegans (C. elegans) is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin MPP+ enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research.
语种英语
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WOS记录号WOS:000273217500014
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/102791
专题中国科学院大连化学物理研究所
通讯作者Qin JH(秦建华); Lin BC(林炳承)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
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GB/T 7714
Ma, Hui,Jiang, Lei,Shi, Weiwei,et al. A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans[J]. BIOMICROFLUIDICS,2009,3(4):044114-1-044114-8.
APA Ma, Hui.,Jiang, Lei.,Shi, Weiwei.,Qin, Jianhua.,Lin, Bingcheng.,...&林炳承.(2009).A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans.BIOMICROFLUIDICS,3(4),044114-1-044114-8.
MLA Ma, Hui,et al."A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans".BIOMICROFLUIDICS 3.4(2009):044114-1-044114-8.
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