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题名: A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans
作者: Ma, Hui1;  Jiang, Lei1;  Shi, Weiwei1;  Qin, Jianhua1;  Lin, Bingcheng1
通讯作者: 秦建华 ;  林炳承
关键词: bioMEMS ;  diseases ;  microchannel flow ;  neurophysiology
刊名: BIOMICROFLUIDICS
发表日期: 2009-10-01
DOI: 10.1063/1.3274313
卷: 3, 期:4, 页:044114-1-044114-8
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biophysics ;  Nanoscience & Nanotechnology ;  Physics, Fluids & Plasmas
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics ;  Science & Technology - Other Topics ;  Physics
英文摘要: The soil dwelling nematode Caenorhabditis elegans (C. elegans) is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin MPP+ enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research.
关键词[WOS]: CAENORHABDITIS-ELEGANS ;  ON-CHIP ;  SYSTEM ;  IMMOBILIZATION ;  GENETICS ;  NEURONS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000273217500014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102791
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Ma, Hui,Jiang, Lei,Shi, Weiwei,et al. A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans[J]. BIOMICROFLUIDICS,2009,3(4):044114-1-044114-8.
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