DICP OpenIR
A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans
Ma, Hui; Jiang, Lei; Shi, Weiwei; Qin, Jianhua; Lin, Bingcheng; Qin JH(秦建华); Lin BC(林炳承)
KeywordBiomems Diseases Microchannel Flow Neurophysiology
Source PublicationBIOMICROFLUIDICS
2009-10-01
DOI10.1063/1.3274313
Volume3Issue:4Pages:044114-1-044114-8
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biophysics ; Nanoscience & Nanotechnology ; Physics, Fluids & Plasmas
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Science & Technology - Other Topics ; Physics
WOS KeywordCAENORHABDITIS-ELEGANS ; ON-CHIP ; SYSTEM ; IMMOBILIZATION ; GENETICS ; NEURONS
AbstractThe soil dwelling nematode Caenorhabditis elegans (C. elegans) is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin MPP+ enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research.
Language英语
URL查看原文
WOS IDWOS:000273217500014
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/102791
Collection中国科学院大连化学物理研究所
Corresponding AuthorQin JH(秦建华); Lin BC(林炳承)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Ma, Hui,Jiang, Lei,Shi, Weiwei,et al. A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans[J]. BIOMICROFLUIDICS,2009,3(4):044114-1-044114-8.
APA Ma, Hui.,Jiang, Lei.,Shi, Weiwei.,Qin, Jianhua.,Lin, Bingcheng.,...&林炳承.(2009).A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans.BIOMICROFLUIDICS,3(4),044114-1-044114-8.
MLA Ma, Hui,et al."A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans".BIOMICROFLUIDICS 3.4(2009):044114-1-044114-8.
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