中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Microchip free flow planar reversed phase electrochromatography with monolithic stationary phase
作者: Wang, Pingli1, 2;  Tao, Dingyin1, 2;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Dye ;  Free flow planar electrochromatography ;  Microchip ;  Monolith ;  Protein
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-08-01
DOI: 10.1002/jssc.200900223
卷: 32, 期:15-16, 页:2629-2634
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: In this study, microchip free flow planar RP electrochromatography (mu FF-PRPEC) was developed by in situ polymerization of monolithic materials in microchamber, and successfully applied for the separation of dyes and proteins. Poly(butyle methyacrylate-co-ethylene dimethacrylate) was prepared by UV-initiated polymerization in a glass microchamber (42 mm long, 23 mm wide, and 28 pm. deep). A mixture of 1-propanol, 1,4-butanediol, and water was chosen as porogens, and 1.2% (wt%) 2-acrylamide-2-methyl-propanesulfonic acid (AMPS) was added into the polymerization solution to generate EOF. With 30% v/v ACN-15 mM Tris-HCl as the mobile phase, rhodamine B and methyl green were separated from each other with 400 V transverse voltage applied, and resolution as high as 4.6 was obtained, much higher than that obtained by mu FFE under optimal conditions. Furthermore, mu FF-PRPEC was also successfully applied into the separation of lysozyme and ribonuclease B, and resolution as high as 9.4 was obtained. All these results demonstrate that mu FF-RPPEC might have great potential in the microscale continuous preparation of samples with improved resolution compared to mu FFE.
关键词[WOS]: CAPILLARY ELECTROCHROMATOGRAPHY ;  MICROFLUIDIC DEVICE ;  POROUS POLYMER ;  SEPARATION ;  ELECTROPHORESIS ;  CHROMATOGRAPHY ;  APPARATUS ;  PEPTIDES ;  PROTEINS ;  CHIP
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000269413400017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102793
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Wang, Pingli,Tao, Dingyin,Zhang, Lihua,et al. Microchip free flow planar reversed phase electrochromatography with monolithic stationary phase[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(15-16):2629-2634.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang, Pingli]'s Articles
 [Tao, Dingyin]'s Articles
 [Zhang, Lihua]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang, Pingli]‘s Articles
 [Tao, Dingyin]‘s Articles
 [Zhang, Lihua]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace