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题名: Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1
作者: Elowe, Sabine1;  Dulla, Kalyan1;  Uldschmid, Andreas1;  Li, Xiuling2;  Dou, Zhen1;  Nigg, Erich A.1, 3
通讯作者: Sabine Elowe
关键词: BubR1 ;  Chromosome congression ;  Kinetochore ;  Mitosis ;  Spindle checkpoint
刊名: JOURNAL OF CELL SCIENCE
发表日期: 2010
DOI: 10.1242/jcs.056507
卷: 123, 期:1, 页:84-94
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 2;4
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
英文摘要: The BubR1 checkpoint protein performs multiple functions in mitosis. We have carried out a functional analysis of conserved motifs of human BubR1 (also known as BUB1B) and demonstrate that spindle assembly checkpoint ( SAC) and chromosome attachment functions can be uncoupled from each other. Mutation of five proline-directed serine phosphorylation sites, identified in vivo by mass spectrometry, essentially abolishes attachment of chromosomes to the spindle but has no effect on SAC functionality. By contrast, mutation of the two conserved KEN boxes required for SAC function does not impact chromosome congression. Interestingly, the contribution of the two KEN-box motifs is not equal. Cdc20 associates with the N-terminal but not C-terminal KEN box, and mutation of the N-terminal KEN motif results in more severe acceleration of mitotic timing. Moreover, the two KEN motifs are not sufficient for maximal binding of Cdc20 and APC/C, which also requires sequences in the BubR1 C-terminus. Finally, mutation of the GLEBS motif causes loss of Bub3 interaction and mislocalization of BubR1 from the kinetochore; concomitantly, BubR1 phosphorylation as well as SAC activity and chromosome congression are impaired, indicating that the GLEBS motif is strictly required for both major functions of human BubR1.
关键词[WOS]: MITOTIC CHECKPOINT ;  ASSEMBLY CHECKPOINT ;  PROTEIN BUBR1 ;  PSEUDOSUBSTRATE INHIBITOR ;  UNATTACHED KINETOCHORES ;  ALIGNMENT ;  ANAPHASE ;  CDC20 ;  MAD2 ;  MITOSIS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000272839800010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/102847
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany
2.Chinese Acad Sci, Dalian Inst Chem Phys, Grp 1803, Dalian 116023, Peoples R China
3.Univ Basel, Biozentrum, CH-4056 Basel, Switzerland

Recommended Citation:
Elowe, Sabine,Dulla, Kalyan,Uldschmid, Andreas,et al. Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1[J]. JOURNAL OF CELL SCIENCE,2010,123(1):84-94.
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