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题名: Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor
作者: Zhou, Houjiang1, 2, 3, 5;  Elisma, Fred2, 3;  Denis, Nicholas J.2, 3;  Wright, Theodore G.2, 3;  Tian, Ruijun2, 3;  Zhou, Hu2, 3;  Hou, Weimin2, 4;  Zou, Hanfa1, 5;  Figeys, Daniel2, 3, 4
通讯作者: 邹汉法 ;  Daniel Figeys
关键词: Phosphoproteomics ;  proteomic reactor ;  subcellular ;  technology
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2010-03-01
DOI: 10.1021/pr900767j
卷: 9, 期:3, 页:1279-1288
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 3;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Protein phosphorylation is an important post-translational modification involved in the regulation of many cellular processes. Mass spectrometry has been successfully used to identify protein phosphorylation in specific pathways and for global phosphoproteomic analysis. However, phosphoproteomics approaches do not evaluate the subcellular localization of the phosphorylated forms of proteins, which is an important factor for understanding the roles of protein phosphorylation on a global scale. The in-depth mapping of protein phosphorylation at the subcellular level necessitates the development of new methods capable of specifically and efficiently enriching phosphopeptides from highly complex samples. Here, we report a novel microfluidic device called the phosphoproteomic reactor that combines efficient processing of proteins followed by phosphopeptide enrichment by Ti-IMAC. To illustrate the potential of this novel technology, we mapped the phosphoproteins in subcellular organelles of liver cells. Fifteen subcellular fractions from liver cell cultures were processed on the phosphoproteomic reactor in combination with nano-LC-MS/MS analysis. We identified thousands of phosphorylation sites in over 600 phosphoproteins in different organelles using minute amounts of starting material. Overall, this approach provides a new avenue for studying the phosphoproteome of the subcellular organelles.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  HIGHLY SELECTIVE ENRICHMENT ;  CYTOSCAPE PLUG-IN ;  MASS-SPECTROMETRY ;  PHOSPHORYLATED PEPTIDES ;  PROTEOMIC REACTOR ;  QUANTITATIVE PHOSPHOPROTEOMICS ;  PHOSPHOPEPTIDE ENRICHMENT ;  ORGANELLE PROTEOMICS ;  TITANIUM-DIOXIDE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000275088100011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103065
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Ottawa, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada
3.Univ Ottawa, Dept Biochem Microbiol & Immunol, Fac Med, Ottawa, ON K1H 8M5, Canada
4.Univ Ottawa, Dept Chem, Ottawa, ON K1H 8M5, Canada
5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Zhou, Houjiang,Elisma, Fred,Denis, Nicholas J.,et al. Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(3):1279-1288.
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