DICP OpenIR
Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor
Zhou, Houjiang1,2,3,5; Elisma, Fred2,3; Denis, Nicholas J.2,3; Wright, Theodore G.2,3; Tian, Ruijun2,3; Zhou, Hu2,3; Hou, Weimin2,4; Zou, Hanfa1,5; Figeys, Daniel2,3,4; Zou HF(邹汉法); Daniel Figeys
关键词Phosphoproteomics Proteomic Reactor Subcellular Technology
刊名JOURNAL OF PROTEOME RESEARCH
2010-03-01
ISSN1535-3893
DOI10.1021/pr900767j
9期:3页:1279-1288
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名3;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]ION AFFINITY-CHROMATOGRAPHY ; HIGHLY SELECTIVE ENRICHMENT ; CYTOSCAPE PLUG-IN ; MASS-SPECTROMETRY ; PHOSPHORYLATED PEPTIDES ; PROTEOMIC REACTOR ; QUANTITATIVE PHOSPHOPROTEOMICS ; PHOSPHOPEPTIDE ENRICHMENT ; ORGANELLE PROTEOMICS ; TITANIUM-DIOXIDE
英文摘要Protein phosphorylation is an important post-translational modification involved in the regulation of many cellular processes. Mass spectrometry has been successfully used to identify protein phosphorylation in specific pathways and for global phosphoproteomic analysis. However, phosphoproteomics approaches do not evaluate the subcellular localization of the phosphorylated forms of proteins, which is an important factor for understanding the roles of protein phosphorylation on a global scale. The in-depth mapping of protein phosphorylation at the subcellular level necessitates the development of new methods capable of specifically and efficiently enriching phosphopeptides from highly complex samples. Here, we report a novel microfluidic device called the phosphoproteomic reactor that combines efficient processing of proteins followed by phosphopeptide enrichment by Ti-IMAC. To illustrate the potential of this novel technology, we mapped the phosphoproteins in subcellular organelles of liver cells. Fifteen subcellular fractions from liver cell cultures were processed on the phosphoproteomic reactor in combination with nano-LC-MS/MS analysis. We identified thousands of phosphorylation sites in over 600 phosphoproteins in different organelles using minute amounts of starting material. Overall, this approach provides a new avenue for studying the phosphoproteome of the subcellular organelles.
语种英语
原文出处查看原文
WOS记录号WOS:000275088100011
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103065
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Daniel Figeys
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Ottawa, Ottawa Inst Syst Biol, Ottawa, ON K1H 8M5, Canada
3.Univ Ottawa, Dept Biochem Microbiol & Immunol, Fac Med, Ottawa, ON K1H 8M5, Canada
4.Univ Ottawa, Dept Chem, Ottawa, ON K1H 8M5, Canada
5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Houjiang,Elisma, Fred,Denis, Nicholas J.,et al. Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(3):1279-1288.
APA Zhou, Houjiang.,Elisma, Fred.,Denis, Nicholas J..,Wright, Theodore G..,Tian, Ruijun.,...&Daniel Figeys.(2010).Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor.JOURNAL OF PROTEOME RESEARCH,9(3),1279-1288.
MLA Zhou, Houjiang,et al."Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor".JOURNAL OF PROTEOME RESEARCH 9.3(2010):1279-1288.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhou, Houjiang]的文章
[Elisma, Fred]的文章
[Denis, Nicholas J.]的文章
百度学术
百度学术中相似的文章
[Zhou, Houjiang]的文章
[Elisma, Fred]的文章
[Denis, Nicholas J.]的文章
必应学术
必应学术中相似的文章
[Zhou, Houjiang]的文章
[Elisma, Fred]的文章
[Denis, Nicholas J.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。