DICP OpenIR
Systematic screening and characterization of novel bufadienolides from toad skin using ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry
Liu, Yanfang; Xiao, Yuansheng; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao; Liang XM(梁鑫淼); Zhang XL(张秀莉)
刊名RAPID COMMUNICATIONS IN MASS SPECTROMETRY
2010-03-01
DOI10.1002/rcm.4436
24期:5页:667-678
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Analytical ; Spectroscopy
研究领域[WOS]Chemistry ; Spectroscopy
关键词[WOS]DRUG CHAN-SU ; CARTHAMUS-TINCTORIUS L. ; POISON BUFADIENOLIDES ; NATURAL-PRODUCTS ; RAT PLASMA ; TOF-MS/MS ; IDENTIFICATION ; DEREPLICATION ; CONSTITUENTS ; ALKALOIDS
英文摘要During the discovery process of novel compounds, it is of significant importance to differentiate novel from known compounds in crude extracts before starting the time-consuming process of purification. Bufadienolides are the main active components of the skin of the toad Bufo bufo gargarizans Cantor (toad skin), an important traditional Chinese medicine. The fragmentation behavior and mass spectra profiles of bufadienolides standards were investigated using ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOFMS). Several fragmentation rules were summarized and applied to characterize novel and known bufadienolides in toad skin. Characteristic substituent groups could be identified by both diagnostic ions and their relative abundance. Bufadienolide stereoisomers could be differentiated from positional isomers by comparing fragment abundance profiles. This was used to characterize new stereoisomers; for known bufadienolides. A total of 39 bufadienolides were screened out using a systematic method developed in our laboratory. In addition to 19 known bufadienolides, 20 putative novel compounds, including 8 stereoisomers, were characterized. UPLC/Q-TOFMS was demonstrated to be a powerful tool for the characterization of low-abundance bufadienolides in complex samples. This study provides guidelines for the targeted isolation of novel bufadienolides from natural products. Copyright (C) 2010 John Wiley & Sons, Ltd.
语种英语
原文出处查看原文
WOS记录号WOS:000275918700026
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103073
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼); Zhang XL(张秀莉)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yanfang,Xiao, Yuansheng,Xue, Xingya,et al. Systematic screening and characterization of novel bufadienolides from toad skin using ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2010,24(5):667-678.
APA Liu, Yanfang.,Xiao, Yuansheng.,Xue, Xingya.,Zhang, Xiuli.,Liang, Xinmiao.,...&张秀莉.(2010).Systematic screening and characterization of novel bufadienolides from toad skin using ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,24(5),667-678.
MLA Liu, Yanfang,et al."Systematic screening and characterization of novel bufadienolides from toad skin using ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 24.5(2010):667-678.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Yanfang]的文章
[Xiao, Yuansheng]的文章
[Xue, Xingya]的文章
百度学术
百度学术中相似的文章
[Liu, Yanfang]的文章
[Xiao, Yuansheng]的文章
[Xue, Xingya]的文章
必应学术
必应学术中相似的文章
[Liu, Yanfang]的文章
[Xiao, Yuansheng]的文章
[Xue, Xingya]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。