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Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites
Shi, Yan1; Han, Guanghui2; Wu, Huiling1; Ye, Kan1; Tian, Zhipeng1; Wang, Jiaqi1; Shi, Huili1; Ye, Mingliang2; Zou, Hanfa2; Huo, Keke1; Ye ML(叶明亮); Keke Huo
关键词Ck2 Mass Spectrometry Msk1 Phosphorylation Yeast Two-hybrid
刊名BMB REPORTS
2009-12-31
42期:12页:840-845
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名2;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]CREB ; CK2 ; SUBUNITS ; MEDIATE ; SEARCH
英文摘要Mitogen- and stress-activated protein kinase (MSK1) palys; a crucial role in the regulation of transcription downstream of extracellular-signal-regulated kinase1/2 (ERK1/2) and mitogen-activated protein kinase p38. MSK1 can be phosphorylated and activated in cells by both ERK1/2 and p38 alpha. In this study, Casein Kinase 2 (CK2) was identified as a binding and regulatory partner for MSK1. Using the yeast two-hybrid system, MSK1 was found to interact with the CK2 beta regulatory subunit of CK2. Interactions between MSK1 and the CK2 alpha catalytic subunit and CK2 beta subunit were demonstrated in vitro and in vivo. We further found that CK2 alpha can only interact with the C-terminal kinase domain of MSK1. Using site-directed mutagenesis assay and mass spectrometry, we identified five sites in the MSK1 C-terminus that could be phosphorylated by CK2 in vitro: Ser757, Ser758, Ser759, Ser760 and Thr793. Of these, Ser757, Ser759, Ser760 and Thr793 were previously unknown. [BMB reports 2009; 42(12): 840-845]
语种英语
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WOS记录号WOS:000273334900012
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103083
专题中国科学院大连化学物理研究所
通讯作者Ye ML(叶明亮); Keke Huo
作者单位1.Fudan Univ, Inst Genet, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200433, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Shi, Yan,Han, Guanghui,Wu, Huiling,et al. Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites[J]. BMB REPORTS,2009,42(12):840-845.
APA Shi, Yan.,Han, Guanghui.,Wu, Huiling.,Ye, Kan.,Tian, Zhipeng.,...&Keke Huo.(2009).Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites.BMB REPORTS,42(12),840-845.
MLA Shi, Yan,et al."Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites".BMB REPORTS 42.12(2009):840-845.
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