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题名: Effect of surface wettability and charge on protein adsorption onto implantable alginate-chitosan-alginate microcapsule surfaces
作者: Xie, Hong-Guo1, 2;  Li, Xiao-Xia1, 2;  Lv, Guo-Jun1;  Xie, Wei-Yang1;  Zhu, Jing1;  Luxbacher, Thomas3;  Ma, Ron4;  Ma, Xiao-Jun1
通讯作者: 马小军
关键词: microcapsule ;  protein adsorption ;  surface charge ;  fibrinogen ;  gamma globulin
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2010-03-15
DOI: 10.1002/jbm.a.32437
卷: 92A, 期:4, 页:1357-1365
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: Alginate-chitosan-alginate (ACA) microcapsules have been developed as a device for the transplantation of living cells. However, protein adsorption onto the surface of microcapsules immediately upon their implantation decides their ultimate biocompatibility. In this work, the chemical composition of the ACA membranes was determined using X-ray photoelectron spectroscopy (XPS). The surface wettability and charge were determined by contact angle and zeta potential measurements, respectively. Then, the effects of surface wettability and charge on bovine fibrinogen (Fgn) and gamma globulin (IgG) adsorption onto ACA microcapsules were evaluated. The results showed that ACA microcapsules had a hydrophilic membrane. So, the surface wettability of ACA microcapsules had little effect on protein adsorption. There was a negative zeta potential of ACA microcapsules which varies with the viscosity or G content of alginate used, indicating a varying degree of net negatively charged groups on the surface of ACA microcapsules. The amount of adsorbed protein increased with increasing of positive charge. Furthermore, the interaction between proteins and ACA microcapsules is dominated by electrostatic repulsion at pH 7.4 and that is of electrostatic attraction at pH 6.0. This work could help to explain the bioincompatibility of ACA microcapsules and will play an important role in the optimization of the microcapsule design. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 92A: 1357-1365, 2010
关键词[WOS]: SOLID-SURFACES ;  OXIDE NANOPARTICLES ;  PLL CAPSULES ;  BIOCOMPATIBILITY ;  ADHESION ;  BEHAVIOR ;  MICROENCAPSULATION ;  TRANSPLANTATION ;  CONFORMATIONS ;  CELLS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000274541500014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103103
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Anton Paar GmbH, A-8054 Graz, Austria
4.Anton Paar China, Beijing 100020, Peoples R China

Recommended Citation:
Xie, Hong-Guo,Li, Xiao-Xia,Lv, Guo-Jun,et al. Effect of surface wettability and charge on protein adsorption onto implantable alginate-chitosan-alginate microcapsule surfaces[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2010,92A(4):1357-1365.
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