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Separation of peptide diastereomers using CEC and a hydrophobic monolithic column
Ludewig, Ronny1; Dong, Jing2; Zou, Hanfa2; Scriba, Gerhard K. E.1; Zou HF(邹汉法)
关键词Cec Neutral Hydrophobic Monolith Peptide Diastereomers Peptide Separation
刊名JOURNAL OF SEPARATION SCIENCE
2010-04-01
ISSN1615-9306
DOI10.1002/jssc.200900739
33期:8页:1085-1089
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名2;2
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]CAPILLARY-ELECTROPHORESIS ; STATIONARY PHASES ; ELECTROCHROMATOGRAPHY ; TRIPEPTIDES ; STEREOISOMERS ; DEGRADATION ; RESOLUTION ; CONSTANTS ; PRODUCTS
英文摘要A neutral hydrophobic monolith prepared by radical in situ copolymerization of lauryl methacrylate and ethylene dimethacylate has been evaluated for the CEC separation of diastereomers of small peptides using acidic mobile phases containing ACN as organic modifier. Using an acidic mobile phase, the peptides migrated due to their own electrophoretic mobility. Hydrophobic interactions with the stationary phase contributed to the separation. Peptide mobility and resolution increased with increasing the ACN content. Retention times increased with the pH of the mobile phase. Peak resolution increased with buffer pH and concentration. Di- and tripeptides composed only of L-configured amino acids migrated faster than peptides containing D-amino acids. A mixture of isomeric Asp tripeptides that could not be completely resolved by either CZE or H PLC as well as the 24mer peptides tetracosactide and (16)[D-Lys]-tetracosactide could also be separated by CEC on the hydrophobic monolith.
语种英语
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WOS记录号WOS:000277248400013
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103119
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
作者单位1.Univ Jena, Dept Pharmaceut & Med Chem, Sch Pharm, D-07743 Jena, Germany
2.Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China
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Ludewig, Ronny,Dong, Jing,Zou, Hanfa,et al. Separation of peptide diastereomers using CEC and a hydrophobic monolithic column[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(8):1085-1089.
APA Ludewig, Ronny,Dong, Jing,Zou, Hanfa,Scriba, Gerhard K. E.,&邹汉法.(2010).Separation of peptide diastereomers using CEC and a hydrophobic monolithic column.JOURNAL OF SEPARATION SCIENCE,33(8),1085-1089.
MLA Ludewig, Ronny,et al."Separation of peptide diastereomers using CEC and a hydrophobic monolithic column".JOURNAL OF SEPARATION SCIENCE 33.8(2010):1085-1089.
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