DICP OpenIR
Separation of peptide diastereomers using CEC and a hydrophobic monolithic column
Ludewig, Ronny1; Dong, Jing2; Zou, Hanfa2; Scriba, Gerhard K. E.1; Zou HF(邹汉法)
KeywordCec Neutral Hydrophobic Monolith Peptide Diastereomers Peptide Separation
Source PublicationJOURNAL OF SEPARATION SCIENCE
2010-04-01
ISSN1615-9306
DOI10.1002/jssc.200900739
Volume33Issue:8Pages:1085-1089
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank2;2
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordCAPILLARY-ELECTROPHORESIS ; STATIONARY PHASES ; ELECTROCHROMATOGRAPHY ; TRIPEPTIDES ; STEREOISOMERS ; DEGRADATION ; RESOLUTION ; CONSTANTS ; PRODUCTS
AbstractA neutral hydrophobic monolith prepared by radical in situ copolymerization of lauryl methacrylate and ethylene dimethacylate has been evaluated for the CEC separation of diastereomers of small peptides using acidic mobile phases containing ACN as organic modifier. Using an acidic mobile phase, the peptides migrated due to their own electrophoretic mobility. Hydrophobic interactions with the stationary phase contributed to the separation. Peptide mobility and resolution increased with increasing the ACN content. Retention times increased with the pH of the mobile phase. Peak resolution increased with buffer pH and concentration. Di- and tripeptides composed only of L-configured amino acids migrated faster than peptides containing D-amino acids. A mixture of isomeric Asp tripeptides that could not be completely resolved by either CZE or H PLC as well as the 24mer peptides tetracosactide and (16)[D-Lys]-tetracosactide could also be separated by CEC on the hydrophobic monolith.
Language英语
URL查看原文
WOS IDWOS:000277248400013
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/103119
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法)
Affiliation1.Univ Jena, Dept Pharmaceut & Med Chem, Sch Pharm, D-07743 Jena, Germany
2.Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Ludewig, Ronny,Dong, Jing,Zou, Hanfa,et al. Separation of peptide diastereomers using CEC and a hydrophobic monolithic column[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(8):1085-1089.
APA Ludewig, Ronny,Dong, Jing,Zou, Hanfa,Scriba, Gerhard K. E.,&邹汉法.(2010).Separation of peptide diastereomers using CEC and a hydrophobic monolithic column.JOURNAL OF SEPARATION SCIENCE,33(8),1085-1089.
MLA Ludewig, Ronny,et al."Separation of peptide diastereomers using CEC and a hydrophobic monolithic column".JOURNAL OF SEPARATION SCIENCE 33.8(2010):1085-1089.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ludewig, Ronny]'s Articles
[Dong, Jing]'s Articles
[Zou, Hanfa]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ludewig, Ronny]'s Articles
[Dong, Jing]'s Articles
[Zou, Hanfa]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ludewig, Ronny]'s Articles
[Dong, Jing]'s Articles
[Zou, Hanfa]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.