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Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes
Wang, Chen1,2,4; Zhou, Jiangrui3; Wang, Shuowen1,4; Ye, Mingliang2; Fan, Guorong1,4; Zou, Hanfa2; Jiang, Chunlei3; Zou HF(邹汉法)
关键词2d-nano-lc Proteomics Tetrahydropalmatine Toxicity
刊名TOXICOLOGY LETTERS
2010-04-15
ISSN0378-4274
DOI10.1016/j.toxlet.2010.01.014
194期:1-2页:8-15
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名2;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]INDUCED LIVER-INJURY ; JIN BU HUAN ; DRUG-INDUCED HEPATOTOXICITY ; L-02 CELLS ; CANCER CELLS ; PROTEIN ; MECHANISMS ; EXPRESSION ; GROWTH ; MTOR
英文摘要The analgesic agent levo-tetrahydropalmatine (l-THP) was reported to be associated with acute or chronic hepatitis in clinical practice. We found that l-THP can induce apoptosis in the hepatocytes of BALB/c mice and human normal liver L-02 (L-02) cells. Several key molecules, including caspase-3, Bcl-2. BAD and Bax, were modulated by l-THP treatment. A novel high-throughput proteomic approach based on 2D-nano-LC-MS/MS was applied to simultaneously evaluate the alterations of global protein expression involved in the response of l-THP treatment in L-02 cells. A total of 156 deregulated proteins were identified, among which 12 proteins play regulatory or constitutive roles in the apoptosis pathways. Further analyses of two proteins (mTOR and MEK2) by Western Blots confirmed that these proteins were expressed at lower levels in l-THP-treated L-02 cells compared with those of control. The current study provided detailed evidence to support that l-THP is capable of inducing apoptosis in mammalian liver cells and improve the understanding of mechanisms of l-THP-induced hepatotoxicity. Crown Copyright (C) 2010 Published by Elsevier Ireland Ltd. All rights reserved.
语种英语
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WOS记录号WOS:000276933400002
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103121
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
作者单位1.Second Mil Med Univ, Sch Pharm, Dept Pharmaceut Anal, Shanghai 200433, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Second Mil Med Univ, Dept Naut Med, Lab Stress Med, Shanghai 200433, Peoples R China
4.Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China
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GB/T 7714
Wang, Chen,Zhou, Jiangrui,Wang, Shuowen,et al. Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes[J]. TOXICOLOGY LETTERS,2010,194(1-2):8-15.
APA Wang, Chen.,Zhou, Jiangrui.,Wang, Shuowen.,Ye, Mingliang.,Fan, Guorong.,...&邹汉法.(2010).Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes.TOXICOLOGY LETTERS,194(1-2),8-15.
MLA Wang, Chen,et al."Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes".TOXICOLOGY LETTERS 194.1-2(2010):8-15.
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