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题名: Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes
作者: Wang, Chen1, 2, 4;  Zhou, Jiangrui3;  Wang, Shuowen1, 4;  Ye, Mingliang2;  Fan, Guorong1, 4;  Zou, Hanfa2;  Jiang, Chunlei3
通讯作者: 邹汉法
关键词: 2D-nano-LC ;  Proteomics ;  Tetrahydropalmatine ;  Toxicity
刊名: TOXICOLOGY LETTERS
发表日期: 2010-04-15
DOI: 10.1016/j.toxlet.2010.01.014
卷: 194, 期:1-2, 页:8-15
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
英文摘要: The analgesic agent levo-tetrahydropalmatine (l-THP) was reported to be associated with acute or chronic hepatitis in clinical practice. We found that l-THP can induce apoptosis in the hepatocytes of BALB/c mice and human normal liver L-02 (L-02) cells. Several key molecules, including caspase-3, Bcl-2. BAD and Bax, were modulated by l-THP treatment. A novel high-throughput proteomic approach based on 2D-nano-LC-MS/MS was applied to simultaneously evaluate the alterations of global protein expression involved in the response of l-THP treatment in L-02 cells. A total of 156 deregulated proteins were identified, among which 12 proteins play regulatory or constitutive roles in the apoptosis pathways. Further analyses of two proteins (mTOR and MEK2) by Western Blots confirmed that these proteins were expressed at lower levels in l-THP-treated L-02 cells compared with those of control. The current study provided detailed evidence to support that l-THP is capable of inducing apoptosis in mammalian liver cells and improve the understanding of mechanisms of l-THP-induced hepatotoxicity. Crown Copyright (C) 2010 Published by Elsevier Ireland Ltd. All rights reserved.
关键词[WOS]: INDUCED LIVER-INJURY ;  JIN BU HUAN ;  DRUG-INDUCED HEPATOTOXICITY ;  L-02 CELLS ;  CANCER CELLS ;  PROTEIN ;  MECHANISMS ;  EXPRESSION ;  GROWTH ;  MTOR
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000276933400002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103121
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Second Mil Med Univ, Sch Pharm, Dept Pharmaceut Anal, Shanghai 200433, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Second Mil Med Univ, Dept Naut Med, Lab Stress Med, Shanghai 200433, Peoples R China
4.Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China

Recommended Citation:
Wang, Chen,Zhou, Jiangrui,Wang, Shuowen,et al. Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes[J]. TOXICOLOGY LETTERS,2010,194(1-2):8-15.
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