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题名: Combined Comparative and Chemical Proteomics on the Mechanisms of levo-Tetrahydropalmatine-Induced Antinociception in the Formalin Test
作者: Wang, Chen1, 2, 4;  Zhou, Jiangrui3;  Wang, Shuowen1, 4;  Ye, Mingliang2;  Jiang, Chunlei3;  Fan, Guorong1, 4;  Zou, Hanfa2
通讯作者: 邹汉法
关键词: 2-D nano LC ;  proteomics ;  chemoproteomics ;  tetrahydropalmatine
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2010-06-01
DOI: 10.1021/pr1001274
卷: 9, 期:6, 页:3225-3234
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: This study investigated the mechanisms involved in the antinociceptive action induced by levo-tetrahydropalmatine (L-THP) in the formalin test by combined comparative and chemical proteomics. Rats were pretreated with L-THP by the oral route (40 mg/kg) 1 h before formalin injection. The antinociceptive effect of L-THP was shown in the first and second phases of the formalin test. To address the mechanisms by which L-THP inhibits formalin-induced nociception in rats, the combined comparative and chemical proteomics were applied. A novel high-throughput comparative proteomic approach based on 2D-nano-LC-MS/MS was applied to simultaneously evaluate the deregulated proteins involved in the response of L-THP treatment in formalin-induced pain rats. Thousands of proteins were identified, among which 17 proteins survived the stringent filter criteria and were further included for functional discussion. Two proteins (Neurabin-1 and Calcium-dependent secretion activator 1) were randomly selected, and their expression levels were further confirmed by Western Blots. The results matched well with those of proteomics. In the present study, we also described the development and application of L-THP immobilized beads to bind the targets. Following incubation with cellular lysates, the proteome interacting with the fixed L-THP was identified. The results of comparative and chemical proteomics were quite complementary. Although the precise roles of these identified moleculars in L-THP-induced antinociception need further study, the combined results indicated that proteins associated with signal transduction, vesicular trafficking and neurotransmitter release, energy metabolism, and ion transport play important roles in L-THP-induced antinociception in the formalin test.
关键词[WOS]: ACTIN-DEPOLYMERIZING PROTEIN ;  DENSE-CORE VESICLES ;  NEUROPATHIC PAIN ;  PHOSPHOINOSITIDE 3-KINASE ;  BIOMARKER DISCOVERY ;  DOPAMINE-RECEPTORS ;  ACTIVATOR PROTEIN ;  FOS EXPRESSION ;  PROTON PUMPS ;  HUMAN-BRAIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000278243300041
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103125
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Second Mil Med Univ, Sch Pharm, Dept Pharmaceut Anal, Shanghai 200433, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Second Mil Med Univ, Dept Naut Med, Lab Stress Med, Shanghai 200433, Peoples R China
4.Shanghai Key Lab Pharmaceut Metabolite, Shanghai 200433, Peoples R China

Recommended Citation:
Wang, Chen,Zhou, Jiangrui,Wang, Shuowen,et al. Combined Comparative and Chemical Proteomics on the Mechanisms of levo-Tetrahydropalmatine-Induced Antinociception in the Formalin Test[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(6):3225-3234.
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