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A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum
Zhu Jun; Wang FangJun; Dong XiaoLi; Ye MingLiang; Zou HanFa; Zou HF(邹汉法)
KeywordPeptidomics Label-free Quantification Hepatocellular Carcinoma Breast Cancer Human Serum
Source PublicationSCIENCE CHINA-CHEMISTRY
2010-04-01
DOI10.1007/s11426-010-0127-7
Volume53Issue:4Pages:759-767
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordMESOPOROUS SILICA PARTICLES ; TANDEM MASS-SPECTROMETRY ; BIOMARKER DISCOVERY ; PROTEOMIC PATTERNS ; CANCER ; PROTEIN ; DISEASE ; IDENTIFICATION ; METABOLISM ; ENRICHMENT
AbstractPeptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using mu UPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on. mu UPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained.
Language英语
URL查看原文
WOS IDWOS:000278263400009
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/103153
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhu Jun,Wang FangJun,Dong XiaoLi,et al. A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum[J]. SCIENCE CHINA-CHEMISTRY,2010,53(4):759-767.
APA Zhu Jun,Wang FangJun,Dong XiaoLi,Ye MingLiang,Zou HanFa,&邹汉法.(2010).A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum.SCIENCE CHINA-CHEMISTRY,53(4),759-767.
MLA Zhu Jun,et al."A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum".SCIENCE CHINA-CHEMISTRY 53.4(2010):759-767.
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