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A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum
Zhu Jun; Wang FangJun; Dong XiaoLi; Ye MingLiang; Zou HanFa; Zou HF(邹汉法)
关键词Peptidomics Label-free Quantification Hepatocellular Carcinoma Breast Cancer Human Serum
刊名SCIENCE CHINA-CHEMISTRY
2010-04-01
DOI10.1007/s11426-010-0127-7
53期:4页:759-767
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]MESOPOROUS SILICA PARTICLES ; TANDEM MASS-SPECTROMETRY ; BIOMARKER DISCOVERY ; PROTEOMIC PATTERNS ; CANCER ; PROTEIN ; DISEASE ; IDENTIFICATION ; METABOLISM ; ENRICHMENT
英文摘要Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using mu UPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on. mu UPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained.
语种英语
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WOS记录号WOS:000278263400009
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103153
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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Zhu Jun,Wang FangJun,Dong XiaoLi,et al. A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum[J]. SCIENCE CHINA-CHEMISTRY,2010,53(4):759-767.
APA Zhu Jun,Wang FangJun,Dong XiaoLi,Ye MingLiang,Zou HanFa,&邹汉法.(2010).A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum.SCIENCE CHINA-CHEMISTRY,53(4),759-767.
MLA Zhu Jun,et al."A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum".SCIENCE CHINA-CHEMISTRY 53.4(2010):759-767.
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