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题名: A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum
作者: Zhu Jun1;  Wang FangJun1;  Dong XiaoLi1;  Ye MingLiang1;  Zou HanFa1
通讯作者: 邹汉法
关键词: peptidomics ;  label-free quantification ;  hepatocellular carcinoma ;  breast cancer ;  human serum
刊名: SCIENCE CHINA-CHEMISTRY
发表日期: 2010-04-01
DOI: 10.1007/s11426-010-0127-7
卷: 53, 期:4, 页:759-767
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using mu UPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on. mu UPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained.
关键词[WOS]: MESOPOROUS SILICA PARTICLES ;  TANDEM MASS-SPECTROMETRY ;  BIOMARKER DISCOVERY ;  PROTEOMIC PATTERNS ;  CANCER ;  PROTEIN ;  DISEASE ;  IDENTIFICATION ;  METABOLISM ;  ENRICHMENT
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000278263400009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103153
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Zhu Jun,Wang FangJun,Dong XiaoLi,et al. A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum[J]. SCIENCE CHINA-CHEMISTRY,2010,53(4):759-767.
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