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Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis
Jiang, Xinning; Ye, Mingliang; Han, Guanghui; Dong, Xiaoli; Zou, Hanfa; Ye ML(叶明亮); Zou HF(邹汉法)
刊名ANALYTICAL CHEMISTRY
2010-07-15
ISSN0003-2700
DOI10.1021/ac100975t
82期:14页:6168-6175
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]DECOY SEARCH STRATEGY ; TANDEM MASS-SPECTRA ; PHOSPHORYLATION ANALYSIS ; PROTEIN IDENTIFICATIONS ; DATABASE SEARCH ; PHOSPHOPEPTIDES ; SPECTROMETRY ; ALGORITHM ; PEPTIDES ; MS/MS
英文摘要Data dependent neutral loss triggered MS3 methodology (NLMS3) is often applied to acquire MS data for the analysis of phosphopeptides. Some phosphopeptides tend to seriously lose the phosphate and result in MS2 spectra with poor fragments and fragment-rich MS3 spectra, while some phosphopeptides do not lose phosphate and result in nice MS2 spectra. Since different phosphopeptides have fragment spectra with different characteristics, filtering all of the phosphopeptide identifications by setting a global filter criteria may be inappropriate and result in low sensitivity. In this study, we developed a classification filtering strategy to improve the phosphopeptide identification and phosphorylation site localization. Phosphopeptide identifications were classified into four classes according to their different characteristics, and then, the identifications from each class of mass spectra were processed and filtered separately using different filtering strategies. It was found that the overlap of phosphopeptide identifications from different classes was low and the classification strategy significantly improved the coverage of the phosphoproteome analysis. Compared with MS2 strategy and multiple stage activation (MSA) strategy, NLMS3 with the classification filtering strategy was demonstrated to have higher sensitivity and higher performance in localizing the phosphorylation to specific sites.
语种英语
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WOS记录号WOS:000279727800034
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/103163
专题中国科学院大连化学物理研究所
通讯作者Ye ML(叶明亮); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Jiang, Xinning,Ye, Mingliang,Han, Guanghui,et al. Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis[J]. ANALYTICAL CHEMISTRY,2010,82(14):6168-6175.
APA Jiang, Xinning.,Ye, Mingliang.,Han, Guanghui.,Dong, Xiaoli.,Zou, Hanfa.,...&邹汉法.(2010).Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis.ANALYTICAL CHEMISTRY,82(14),6168-6175.
MLA Jiang, Xinning,et al."Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis".ANALYTICAL CHEMISTRY 82.14(2010):6168-6175.
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