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题名: Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis
作者: Jiang, Xinning1;  Ye, Mingliang1;  Han, Guanghui1;  Dong, Xiaoli1;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
刊名: ANALYTICAL CHEMISTRY
发表日期: 2010-07-15
DOI: 10.1021/ac100975t
卷: 82, 期:14, 页:6168-6175
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Data dependent neutral loss triggered MS3 methodology (NLMS3) is often applied to acquire MS data for the analysis of phosphopeptides. Some phosphopeptides tend to seriously lose the phosphate and result in MS2 spectra with poor fragments and fragment-rich MS3 spectra, while some phosphopeptides do not lose phosphate and result in nice MS2 spectra. Since different phosphopeptides have fragment spectra with different characteristics, filtering all of the phosphopeptide identifications by setting a global filter criteria may be inappropriate and result in low sensitivity. In this study, we developed a classification filtering strategy to improve the phosphopeptide identification and phosphorylation site localization. Phosphopeptide identifications were classified into four classes according to their different characteristics, and then, the identifications from each class of mass spectra were processed and filtered separately using different filtering strategies. It was found that the overlap of phosphopeptide identifications from different classes was low and the classification strategy significantly improved the coverage of the phosphoproteome analysis. Compared with MS2 strategy and multiple stage activation (MSA) strategy, NLMS3 with the classification filtering strategy was demonstrated to have higher sensitivity and higher performance in localizing the phosphorylation to specific sites.
关键词[WOS]: DECOY SEARCH STRATEGY ;  TANDEM MASS-SPECTRA ;  PHOSPHORYLATION ANALYSIS ;  PROTEIN IDENTIFICATIONS ;  DATABASE SEARCH ;  PHOSPHOPEPTIDES ;  SPECTROMETRY ;  ALGORITHM ;  PEPTIDES ;  MS/MS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000279727800034
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103163
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Jiang, Xinning,Ye, Mingliang,Han, Guanghui,et al. Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis[J]. ANALYTICAL CHEMISTRY,2010,82(14):6168-6175.
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