中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Preparation of phenylboronic acid functionalized cation-exchange monolithic columns for protein separation and refolding
作者: Sun, Xiangli1;  Liu, Rui2;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 3
通讯作者: 陈朗星
关键词: Surface functionalization ;  Phenylboronic acid ;  Cation-exchange chromatography ;  Protein separation ;  Refolding
刊名: TALANTA
发表日期: 2010-05-15
DOI: 10.1016/j.talanta.2010.01.029
卷: 81, 期:3, 页:856-864
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;5
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: In this study, we described a simple and effective modification procedure to prepare poly (methacrylate-co-ethylene glycol dimethacrylate) monolithic columns functionalized with 3-aminophenylboronic acid. The column morphology, pore size and specific surface area of the fabricated monolith were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, thermogravimetric analysis, and mercury intrusion porosimeter, respectively. The frontal analysis was carried out for dynamic loading capacity of the model protein on the modified column. The chromatographic performance of the cation-exchange monolith was evaluated through separating a mixture of five proteins such as lysozyme, cytochrome c, ribonuclease A, trypsin and bovine serum albumin and one-step purification of lysozyme from egg whites, and the expected results were obtained. In addition, the functionalized column was used to refold ribonuclease A and cytochrome c. and this procedure was monitored by circular dichroism and fluorescence spectroscopy. Compared with the conventional dilution refolding method, the ion-exchange chromatography refolding method developed here is more effective for specific bioactivity recovery. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  BORONATE AFFINITY-CHROMATOGRAPHY ;  CAPILLARY ELECTROCHROMATOGRAPHY ;  ION CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  STATIONARY-PHASE ;  TRANSPORT ;  PEPTIDES ;  SENSORS ;  MODE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000276877500018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/103205
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Tianjin Med Univ, Basic Med Coll, Dept Chem, Tianjin 300070, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Sun, Xiangli,Liu, Rui,He, Xiwen,et al. Preparation of phenylboronic acid functionalized cation-exchange monolithic columns for protein separation and refolding[J]. TALANTA,2010,81(3):856-864.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Sun, Xiangli]'s Articles
 [Liu, Rui]'s Articles
 [He, Xiwen]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Sun, Xiangli]‘s Articles
 [Liu, Rui]‘s Articles
 [He, Xiwen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace